Skip to main content
SpringerLink
Log in

Histochemical method for demonstrating aldolase

  • Published:
Histochemie Aims and scope Submit manuscript

Summary

By combing the indirect method of aldolase activity of Warburg and Christian, which consisted in the measurement of reduction of DPN in the presence of glyceraldehyde-3-phosphate dehydrogenase and arsenate, with nitro-BT reduction and we could obtain the much better method of demonstrating aldolase than that of Allen and Bourne.

The optimal incubating mixture was composed of 1) 10 ml 0.02 M sodium fructose-1,6-diphosphate, 2) 5 mg DPN, 3) 10 mg nitro-BT, 4) 10 ml of 0.05 M arsenate-HCl buffer (pH 7.6). Fresh frozen section, which were fixed briefly in 80% cold ethanol, gave a better staining results. The distribution of aldolase of some organs of rat and the validity and limitation of the method were described.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Allen, R. J. L., and G. H. Bourne: Some experiments on the microscopical demonstration of zymohexase in animal tissues. J. exp. Biol. 20, 61–64 (1943).

    Google Scholar 

  • Gomori, T.: Microscopic histoohemistry. Chicago: Chicago University Press 1953.

    Google Scholar 

  • Hashimoto, T.: Histochemical studies on various dehydrogenases related to glucose metabolism of the human skin. Skin Research 3, 245–276 (1961).

    Google Scholar 

  • Hershey, F. B., C. Lewis, Jr., G. Johnston, and J. Murphy: Phosphopyridine nucleotide linked dehydrogenases, aldolase and fumarase in mouse breast. J. Histochem. Cytochem. 11, 224–231 (1963).

    Google Scholar 

  • Himmelhoch, S. R., and M. J. Karnovsky: The histochemical demonstration of glyceraldehyde-3-phosphate dehydrogenase activity. J. biophys. biochem. Cytol. 9, 573–581 (1961).

    Google Scholar 

  • Nachlas, M. M., D. G. Walker, and A. M. Seligman: A histochemical method for the demonstration of diphosphopyridine nucleotide diaphorase. J. biophys. biochem. Cytol. 4, 29–38 (1958).

    Google Scholar 

  • Pearse, A. G. E.: Histochemistry. London: J. & A. Churchill 1960.

    Google Scholar 

  • Sibley, J. A., and A. L. Lehninger: Determination of aldolase in animal tissues. J. biol. Chem. 177, 859–872 (1949).

    Google Scholar 

  • Warburg, O., u. W. Christian: Isolierung und Kristallisation des Gärungsferments Zymohexase. Biochem. Z. 314, 149–176 (1943).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anatomy, Osaka University Medical School, Osaka, Japan

    Tomiya Abe & Nobuo Shimizu

Authors
  1. Tomiya Abe
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nobuo Shimizu
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Abe, T., Shimizu, N. Histochemical method for demonstrating aldolase. Histochemie 4, 209–212 (1964). https://doi.org/10.1007/BF00290865

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00290865

Keywords

Search

Navigation