Abstract
Tomato plants homozygous for the recessive lateral suppressor (ls) mutation show a number of phenotypic abnormalities among which the lack of lateral meristem initiation during vegetative growth and the absence of petals on the flower are the most prominent. As a first step towards the isolation of the Ls gene by means of map-based cloning, we have determined its position on the restriction fragment length polymorphism (RFLP) map of tomato. RFLP analysis of 527 F2 plants segregating for the ls allele allowed us to define an interval of 0.8 cM in which the Ls gene is located. Analysis of the physical distance between the two flanking RFLP markers by pulsed field gel electrophoresis revealed that they lie no further than 375 kb apart. Knowledge of the physical distance together with the availability of a tomato yeast artificial chromosome (YAC) library, makes it feasible to isolate the Ls gene by a map-based cloning approach.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Brandstädter J, Roßbach C, Theres K (1994) The pattern of the histone H4 expression in the tomato shoot apex changes during development. Planta 192:69–74
Chu G, Vollrath D, Davis RW (1986) Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science 234:1582–1585
Churchill GA, Giovannoni JJ, Tanksley SD (1993) Pooled-sampling makes high-resolution mapping practical with DNA markers. Proc Natl Acad Sci USA 90:16–20
Ganal MW, Tanksley SD (1989) Analysis of tomato DNA by pulsed field electrophoresis. Plant Mol Biol Rep 7: 17–27
Ganal MW, Young ND, Tanksley SD (1989) Pulsed field gel electrophoresis and physical mapping of large DNA fragments in the Tm-2a region of chromosome 9 in tomato. Mol Gen Genet 215:395–400
Ganal MW, Bonierbale MW, Röder MS, Park WD, Tanksley SD (1991) Genetic and physical mapping of the patatin genes in potato and tomato. Mol Gen Genet 225:501–509
Groot SPC, Keizer LCP, de Ruiter W, Dons JIM (1994a) Seed and fruit set of the lateral suppressor mutant of tomato. Sci Horti, in press
Groot SPC, Bouwer R, Busscher M, Lindhout P, Dons HJ (1994b) Increase of endogenous zeatin riboside by introduction of the ipt gene in wild type and the lateral suppressor mutant of tomato. Plant Growth Regul, in press
Jones DA, Thomas CM, Hammond-Kosack KE, Balint-Kurti PJ, Jones JDG (1994) Isolation of the tomato Cf-9 gene for resistance to Cladosporium fulvum by transposon tagging. Science 266:789–793
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174–181
Malayer JC, Guard AT (1964) A comparative developmental study of the mutant side-shootless and normal tomato plants. Amer. Jour. Bot. 51:140–143
Maldiney R, Pelèse F, Pilate G, Sossountzov L, Miginiac E (1986) Endogenous levels of abscisic acid, indole-3-acetic acid, zeatin, and zeatin-riboside during the course of adventitious root formation in cuttings of Craigella and Craigella lateral suppressor tomatoes. Physiol Plant 68: 426–430
Martin GB, Ganal MW, Tanksley SD (1992) Construction of a yeast artificial chromosome library of tomato and identification of cloned segments linked to two disease resistance loci. Mol Gen Genet 233:25–32
Martin GB, Brommonschenkel SH, Chungwonse J, Frary A, Ganal MW, Spivey R, Wu T, Earle ED, Tanksley SD (1993) Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science 262: 1432–1436
Segal G, Sarfatti M, Schaffer MA, Ori N, Zamir D, Fluhr R (1992) Correlation of genetic and physical structure in the region surrounding the I 2 Fusarium oxysporum resistance locus in tomato. Mol Gen Genet 231:179–185
Sossountzov L, Maldiney R, Sotta B, Sabbagh I, Habricot Y, Bonnet M, Miginiac E (1988) Immunocytochemical localization of cytokinins in Craigella tomato and a side-shootless mutant. Planta 175:291–304
Tanksley SD, Ganal MW, Prince JP, Vicente MC, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB, Messeguer R, Miller JC, Miller L, Paterson AH, Pineda O, Ršder MS, Wing RA, Wu W, Young ND (1992) High density molecular linkage maps of the tomato and potato genomes. Genetics 132: 1141–1160
Taylor IB, Rossall S (1982) The genetic relationship between the tomato mutants, flacca and lateral suppressor, with reference to abscisic acid accumulation. Planta 154: 1–5
Tucker DJ (1976) Endogenous growth regulators in relation to side shoot development in the tomato. New Phytol 77: 561–568
Williams W (1960) The effect of selection on the manifold expression of the “suppressed lateral” gene in the tomato. Heredity 14:285–296
Woodman KJ (1993) Heterologous transposons for tagging ABA biosynthetic genes in tomato. PhD Thesis, University of Nottingham, Nottingham, UK
Author information
Authors and Affiliations
Additional information
Communicated by H. Saedler
Rights and permissions
About this article
Cite this article
Schumacher, K., Ganal, M. & Theres, K. Genetic and physical mapping of the lateral suppressor (ls) locus in tomato. Molec. Gen. Genet. 246, 761–766 (1995). https://doi.org/10.1007/BF00290724
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00290724