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Actin cytoskeleton and budding pattern are altered in the yeast rvs161 mutant: the Rvs161 protein shares common domains with the brain protein amphiphysin

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Abstract

The actin cytoskeleton cells is altered in rvs161 mutant yeast, with the defect becoming more pronounced under unfavorable growth conditions, as described for the rvs167 mutant. The cytoskeletal alteration has no apparent effect on invertase secretion and polarized growth. Mutations in RTVS161, just as in RI/S167, lead to a random budding pattern in a/α diploid cells. This behavior is not observed in a/a diploid cells homozygous for the rvs161-1 or rvs167-1 mutations. In addition, sequence comparisons revealed that amphiphysin, a protein first found in synaptic vesicles of chicken and shown to be the autoantigen of Stiff Man syndrome, presents similarity with both Rvs proteins. Furthermore, limited similarities with myosin heavy chain and tropomyosin alpha chain from higher eukaryotic cells allow for the definition of a possible consensus sequence. The finding of related sequences suggests the existence of a function for these proteins that is conserved among eukaryotic organisms.

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References

  • Adams AEM, Pringle JR (1984) Relationship of actin and tubulin distribution to bud growth in wild-type and morphogeneticmutant Saccharomyces cerevisiae. J Cell Biol 98:934–945

    Google Scholar 

  • Adams AEM, Johnson DI, Longnecker RM, Sloat BF, Pringle JR (1990) CDC42 and CDC43, two additional genes involved in budding and the establishment of cell polarity in the yeast Saccharomyces cerevisiae. J Cell Biol 111:131–142

    Google Scholar 

  • Amatruda JF, Cannon JF, Tatchell K, Hug C, Cooper JA (1990) Disruption of the actin cytoskeleton in yeast capping protein mutants. Nature 344:352–354

    Google Scholar 

  • Amatruda JF, Gattermeir DJ, Karpova TS, Cooper JA (1992) Effects of null mutations and overexpression of capping protein on morphogenesis, actin distribution and polarized secretion in yeast. J Cell Biol 119:1151–1162

    Google Scholar 

  • Bauer F, Urdaci M, Aigle M, Crouzet M (1993) Alteration of a yeast SH3 protein leads to conditional viability with defects in cytoskeletal and budding patterns. Mol Cell Biol 13:5070–5084

    Google Scholar 

  • Bender A, Pringle JR (1989) Multicopy suppression of the cdc24 budding defect in yeast by CDC42 and three new genes including the ras-related gene RSR1. Proc Natl Acad Sci USA 86:9976–9980

    Google Scholar 

  • Carlson M, Botstein D (1982) Two differentially regulated mRNAs with different 5' ends encode secreted and intracellular forms of yeast invertase. Cell 28:145–154

    Google Scholar 

  • Chant J, Herskowitz I (1991) Genetic control of bud site selection in yeast by a set of gene products that constitute. a morphogenetic pathway. Cell 65:1203–1212

    Google Scholar 

  • Chant J, Corrado K, Pringle JR, Herskowitz I (1991) Yeast BUD5, encoding a putative GDP-GTP exchange factor, is necessary for bud site selection and interacts with bud formation gene BEMI. Cell 65:1213–1224

    Google Scholar 

  • Chenevert J, Corrado K, Bender A, Pringle J, Herskowitz I (1992) A yeast gene (BEM1) necessary for cell polarization whose product contains two SH3 domains. Nature 356:77–79

    Google Scholar 

  • Ciriacy M (1975) Genetics of alcohol dehydrogenase in Saccharomyces cerevisiae. Isolation and genetic analysis of adh mutants. Mutat Res 29:315–326

    Google Scholar 

  • Crouzet M, Urdaci M, Dulau L, Aigle M (1991) Yeast mutant affected for viability upon nutrient starvation: characterization and cloning of the RVS161 gene. Yeast 7:727–743

    Google Scholar 

  • De Camilli P, Thomas A, Cofiel R, Folli F, Lichre B, Piccolo G, Meinck HM, Austoni M, Fassetta G, Bottazzo G, Bates D, Cartlidge N, Solimena M, Kilimann M (1993) The synaptic vesicle-associated protein amphiphysin is the 128-kD autoantigen of Stiff-Man syndrome with breast cancer. J Exp Med 178:2219–2223

    Google Scholar 

  • Desfarges L, Durrens P, Juguelin H, Cassagne C, Bonneu M, Aigle M (1993) Yeast mutants affected in viability upon starvation have a modified phospholipid composition. Yeast 9:267–277

    Google Scholar 

  • Drubin DG (1991) Development of cell polarity in budding yeast. Cell 65:1093–1096

    Google Scholar 

  • Drubin DG, Miller KG, Botstein D (1988) Yeast actin-binding proteins: evidence for a role in morphogenesis. J Cell Biol 107:2551–2561

    Google Scholar 

  • Drubin DG, Mulholland J, Zhu Z, Botstein D (1990) Homology of a yeast actin-binding protein to signal transduction proteins and myosin-I. Nature 343:288–290

    Google Scholar 

  • Field C, Schekman R (1980) Localized secretion of acid phosphatase reflects the pattern of cell surface growth in Saccharomyces cerevisiae. J Cell Biol 86:123–128

    Google Scholar 

  • Freifelder D (1960) Bud position in Saccharomyces cerevisiae. J Bacteriol 80:567–568

    Google Scholar 

  • Goldstein A, Lampen JO (1975) β-d-Fructofuranoside fructo-hydrolase from yeast. Methods Enzymol 42:504–511

    Google Scholar 

  • Haarer BK, Lillie SH, Adams AEM, Magdolen V, Bandlow W, Brown SS (1990) Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells. J Cell Biol 110:105–114

    Google Scholar 

  • Herskowitz I (1989) A regulatory hierarchy for cell specialization in yeast. Nature 342:749–757

    Google Scholar 

  • Johnson DI, Pringle JR (1990) Molecular characterization of CDC42, a Saccharomyces cerevisiae gene involved in the development of cell polarity. J Cell Biol 111:143–152

    Google Scholar 

  • Jung G, Saxe CL III, Kimmel AR, Hammer JA III (1989) Dictyostelium discoideum contains a gene encoding a myosin I heavy chain. Proc Natl Acad Sci USA 86:6186–6190

    Google Scholar 

  • Karlick CC, Fyrberg EA (1986) Two Drosophila melanogaster tropomyosin genes: structural and functional aspects. Mol Cell Biol 6:1965–1973

    Google Scholar 

  • Kiehart DP (1990) Molecular genetic dissection of myosin heavy chain function. Cell 60:347–350

    Google Scholar 

  • Kilmartin JV, Adams AEM (1984) Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces. J Cell Biol 98:922–933

    Google Scholar 

  • Koch CA, Anderson D, Moran MF, Ellis C, Pawson T (1991) SH2 and SH3 domains: elements that control interactions of cytoplasmic signaling proteins. Science 252:668–674

    Google Scholar 

  • Kübler E, Riezman H (1993) Actin and fibrin are required for the internalization step of endocytosis in yeast. EMBO J 12:2855–2862

    Google Scholar 

  • Lew DJ, Reed SI (1993) Morphogenesis in the yeast cell cycle: regulation by Cdc28 and cyclins. J Cell Biol 120:1305–1320

    Google Scholar 

  • Lichte B, Veh RW, Meyer HE, Kilimann MW (1992) Amphiphysin, a novel protein associated with synaptic vesicles. EMBO J 11:2521–2530

    Google Scholar 

  • Liu H, Bretscher A (1989) Purification of tropomyosin from Saccharomyces cerevisiae and identification of related proteins in Schizosaccharomyces and Physarum. Proc Natl Acad Sci USA 86:90–93

    Google Scholar 

  • Madden K, Costigan C, Snyder M (1992) Cell polarity and morphogenesis in Saccharomyces cerevisiae. Trends Cell Biol 2:22–29

    Google Scholar 

  • Matsui Y, Toh-e A (1992) Yeast RHO3 and RHO4 ras superfamily genes are necessary for bud growth, and their defect is suppressed by a high dose of bud formation genes CDC42 and BEMI. Mol Cell Biol 12:5690–5699

    Google Scholar 

  • McLachlan AD, Stewart M (1976) The troponin binding region of tropomyosin. Evidence for a site near residues 197 to 217. J Mol Biol 106:1017–1022

    Google Scholar 

  • Nelson WJ (1992) Regulation of cell surface polarity from bacteria to mammals. Science 258:948–955

    Google Scholar 

  • Novick P, Botstein D (1985) Phenotypic analysis of temperature-sensitive yeast actin mutants. Cell 40:405–416

    Google Scholar 

  • Pawson T (1992) Conviction by genetics. Nature 356:285–286

    Google Scholar 

  • Pringle JR (1991) Staining of bud scars and other cell wall chitin with Calcofluor. Methods Enzymol 194:732–735

    Google Scholar 

  • Rose MD, Price BR, Fink GR (1986) Saccharomyces cerevisiae nuclear fusion requires prior activation by alpha factor. Mol Cell Biol 6:3490–3497

    Google Scholar 

  • Ruggieri R, Bender A, Matsui Y, Powers S, Takai Y, Pringle JR, Matsumoto K (1992) RSR1, a ras-like gene homologous to Krev-1 (smg21 Alrap1 A): role in the development of cell polarity and interactions with the Ras pathway in Saccharomyces cerevisiae. Mol Cell Biol 12:758–766

    Google Scholar 

  • Schekman R, Novick P (1982) The secretory process and yeast cell-surface assembly. In: Strathern JN, Jones EW, Broach JR (eds) The molecular biology of the yeast Saccharomyces, vol. II; metabolism and gene expression. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp 361–398

    Google Scholar 

  • Sloat BF, Adams A, Pringle JR (1981) Roles of the CDC24 gene product in cellular morphogenesis during the Saccharomyces cerevisiae cell cycle. J Cell Biol 89:395–405

    Google Scholar 

  • Tkacz JS, Lampen JO (1972) Wall replication in Saccharomyces species: use of fluorescein-conjugated Concanavalin A to reveal the site of mannan insertion. J Gen Microbiol 72:243–247

    Google Scholar 

  • Tkacz JS, Lampen JO (1973) Surface distribution of invertase in growing Saccharomyces cerevisiae cells. J Bacteriol 113:1073–1075

    Google Scholar 

  • Watts FZ, Miller DM, Orr E (1985) Identification of myosin heavy chain in Saccharomyces cerevisiae. Nature 316:83–85

    Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Génétique, CNRS UPR 9026, Avenue des Facultés, Université de Bordeaux II, 33405, Talence Cedex, France

    Pierre Sivadon, Florian Bauer, Michel Aigle & Marc Crouzet

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  1. Pierre Sivadon
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  2. Florian Bauer
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  3. Michel Aigle
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  4. Marc Crouzet
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Communicated by C. P. Hollenberg

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Sivadon, P., Bauer, F., Aigle, M. et al. Actin cytoskeleton and budding pattern are altered in the yeast rvs161 mutant: the Rvs161 protein shares common domains with the brain protein amphiphysin. Molec. Gen. Genet. 246, 485–495 (1995). https://doi.org/10.1007/BF00290452

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  • DOI: https://doi.org/10.1007/BF00290452

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