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Cloning and characterization of the bgxA gene from Erwinia chrysanthemi D1 which encodes a β-glucosidase/xylosidase enzyme

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Abstract

A β-glucosidase/xylosidase gene from Erwinia chrysanthemi strain D1 was cloned and sequenced. This gene, named bgxA, encodes a ca. 71 kDa protein product which, following removal of the leader peptide, resulted in a ca. 69 kDa mature protein that accumulated in the periplasmic space of E. chrysanthemi strain D1 and Escherichia coli cells expressing the cloned gene. The protein exhibited both β-glucosidase and β-xylosidase activities but gave no detectable activity on xylan or carboxymethyl cellulose. The enzyme was classified as a type 3 glycosyl hydrolase, but was unusual in having a truncated B region at the carboxyl-terminus. Several E. chrysanthemi strains isolated from corn produced the glucosidase/xylosidase activity but not those isolated from dicot plants. However, bgxA marker exchange mutants of strain D1 were not detectably altered in virulence on corn leaves.

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Author notes

  1. Simon Vroemen

    Present address: Dept. of Experimental Zoology, Utrecht University, Padualaan 8, Utrecht, The Netherlands

  2. Jacco Heldens

    Present address: Dept. of Virology, Wageningen Agricultural University, Binnenhaven 11, Wageningen, The Netherlands

Authors and Affiliations

  1. Department of Plant Pathology and Genetics Graduate Group, University of California, 92521, Riverside, CA, USA

    Simon Vroemen, Jacco Heldens, Carol Boyd & Noel T. Keen

  2. Centre de Recherches sur les Macromolecules Vegetales, CNRS, F-38041, Grenoble, France

    Bernard Henrissat

Authors
  1. Simon Vroemen
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  2. Jacco Heldens
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  3. Carol Boyd
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  4. Bernard Henrissat
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  5. Noel T. Keen
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Communicated by A. Kondorosi

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Vroemen, S., Heldens, J., Boyd, C. et al. Cloning and characterization of the bgxA gene from Erwinia chrysanthemi D1 which encodes a β-glucosidase/xylosidase enzyme. Molec. Gen. Genet. 246, 465–477 (1995). https://doi.org/10.1007/BF00290450

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  • DOI: https://doi.org/10.1007/BF00290450

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