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Molecular and General Genetics MGG

, Volume 247, Issue 6, pp 709–715 | Cite as

Isolation of a cDNA from Saccharomyces cerevisiae that encodes a high affinity sulphate transporter at the plasma membrane

  • Frank W. Smith
  • Malcolm J. Hawkesford
  • Ian M. Prosser
  • David T. Clarkson
Original Paper

Abstract

Resistance to selenate and chromate, toxic analogues of sulphate, was used to isolate a mutant of Saccharomyces cerevisiae deficient in the capacity to transport sulphate into the cells. A clone which complements this mutation was isolated from a cDNA library prepared from S. cerevisiae poly(A)+ RNA. This clone contains an insert which is 2775 by in length and has a single open reading frame that encodes a 859 amino acid polypeptide with a molecular mass of 96 kDa. Sequence motifs within the deduced amino acid sequence of this cDNA (SUL1) show homology with conserved areas of sulphate transport proteins from other organisms. Sequence analysis predicts the position of 12 putative membrane spanning domains in SUL1. When the cDNA for SUL1 was expressed in S. cerevisiae, a high affinity sulphate uptake activity (Km = 7.5 ± 0.6 μM for SO 4 2− ) was observed. A genomic mutant of S. cerevisiae in which 1096 by were deleted from the SUL1 coding region was constructed. This mutant was unable to grow on media containing less than 5 mM sulphate unless complemented with a plasmid containing the SUL1 cDNA. We conclude that the SUL1 cDNA encodes a S. cerevisiae high affinity sulphate transporter that is responsible for the transfer of sulphate across the plasma membrane from the external medium.

Key words

Yeast Sulphate transport Sulphate uptake Mutagenesis 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Frank W. Smith
    • 1
  • Malcolm J. Hawkesford
    • 2
  • Ian M. Prosser
    • 2
  • David T. Clarkson
    • 2
  1. 1.Division of Tropical Crops and Pastures, CSIROCunningham LaboratorySt LuciaAustralia
  2. 2.IACR-Long Ashton Research Station, Department of Agricultural SciencesUniversity of BristolBristolUK

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