Abstract
Genetic modifiers of opaque2 convert the soft, starchy endosperm of opaque2 maize mutants to a hard, vitreous phenotype, while maintaining the enhanced lysine content of the grain. Genetic analysis of F2 segregating seeds from crosses of opaque2 by modified opaque2 genotypes indicated that the modifiers are complex traits that act codominantly. We developed two different segregating F2 populations and mapped the modifier loci by restriction fragment length polymorphism (RFLP) analysis. A relationship was found between formation of vitreous endosperm and the locus encoding the gamma-zein storage protein, which maps near the centromere of chromosome 7. Endosperm modification was consistently associated with the presence of two rather than one gamma-zein gene at this locus. A second modifier locus was mapped near the telomere of chromosome 7L.
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References
Beckett JB (1978) B-A translocations in maize. I. Use locating genes by chromosomal arms. J Here 69:27–36
Belousov AA (1987) Genetic analysis of modified endosperm texture in opaque-2 maize. Soviet Genet 23:459–464
Benner MS, Phillips RL, Kirihara JA, Messing JW (1989) Genetic analysis of methionine-rich storage protein accumulation in maize. Theor Appl Genet 78:761–767
Bjarnason M, Vasal SK (1992) Breeding Quality Protein Maize (QPM). Plant Breeding Rev 9:181–216
CIMMYT (1987) CIMMYT report on maize improvement, 1982–1983. CIMMYT, Mexico, D.F.
Dannenhoffer JM, Bostwick DE, Or E, Larkins BA (1995) opaque15, a novel mutation with properties of an opaque2 modifier. Proc Natl Acad Sci USA 92:1931–1935
Das OP, Messing JW (1987) Allelic variation and differential expression at the 27-kilodalton zein locus in maize. Mol Cell Biol 7:4490–4497
Das OP, Minzi SL, Koury M, Benner M, Messing J (1990) A somatic gene rearrangement contributing to genetic diversity in maize. Proc Natl Acad Sci USA 87:7809–7813
Geetha KB, Lending CR, Lopes MA, Wallace JC, Larkins BA (1991) opaque-2 modifiers increase gamma-zein synthesis and alter its spatial distribution in maize endosperm. Plant Cell 3:1207–1219
Geraghty DE (1985) Structure and organization of the zein multigene family. PhD Thesis, University of Minnesota, St. Paul
Gevers HO, Lake JK (1992) Development of modified opaque-2 maize in South Africa. In: Mertz ET (ed) Quality protein maize. The American Association of Cereal Chemists, St. Paul, Minn., 49–78
Kniep KR, Mason SC (1989) Kernel breakage and density of normal and opaque-2 maize grain as influenced by irrigation and nitrogen. Crop Sci 29:158–163
Kramer HH, Burnham CR (1947) Methods of combining linkage intensity values from backcross, F2 and F3 genetic data. Genetics 32:379–390
Kreike CM, Koning JRA, Krens FA (1990) Non-radioactive detection of single-copy DNA-DNA hybrids. Plant Mol Biol Rep 8:172–179
Laemmli UK (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227:680–685
Lopes MA (1993) Genetic and biochemical characterization of the maize mutants floury-2 and modified opaque-2. PhD Thesis, University of Arizona, Tucson
Lopes MA, Larkins BA (1991) Gamma-zein content is related to endosperm modification in quality protein maize (QPM). Crop Sci 31:1655–1662
Lopes MA, Larkins BA (1995) Genetic analysis of opaque-2 modifier gene activity in maize endosperm. Theoret Appl Genet (in press)
McCreery T, Helentjaris T (1994a) Production of hybridization probes by the PCR utilizing digoxigenin-modified nucleotides. In: Isaac P (ed) Protocols for nucleic acid analysis by nonradioactive techniques. Humana Press, Totowa, N.J., pp 107–112
McCreery T, Helentjaris T (1994b) Hybridization of digoxigenin labeled probes to Southern blots and detection by chemiluminescence. In: Isaac P (ed) Protocols for nucleic acid analysis by nonradioactive techniques. Humana Press, Totowa, N.J., pp 107–112
Mertz ET (1992) Quality Protein Maize. American Association of Cereal Chemists, St. Paul, Minn.
Mertz ET, Bates LS, Nelson OE (1964) Mutant that changes protein composition and increases lysine content of maize endopserm. Science 145:279–280
Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832
Or E, Boyer SK, Larkins BA (1993) opaque2 modifiers act posttranscriptionally and in a polar manner on γ-zein gene expression in maize endosperm. Plant Cell 5:1599–1609
Ortega EL, Bates LS (1983) Biochemical and agronomic studies of two modified hard-endosperm opaque2 maize (Zea mays L.) populations. Cereal Chem 60:107–111
Paez AV, Helm JL, Zuber MS (1969) Lysine content of opaque-2 maize kernels having different phenotypes. Crop Sci 9:251–252
Prat S, Cortadas J, Puigdomenech P, Palau J (1985) Nucleic acid (cDNA) and amino acid sequences of the maize endosperm protein glutelin-2. Nucleic Acids Res 13:1493–1504
Reina M, Ponte I, Guillen P, Borant A, Palau J (1990) Sequence analysis of a genomic clone encoding a Zc2 protein from Zea mays W64A. Nucleic Acids Res 18:6426
Saghai-Maroof MA, Soliman KM, Jorgensen R, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Soave C, Reggiani R, Di Fonzo N, Salamini F (1982) Genes for zein subunits on maize chromosome 4. Biochem Genet 20:1027–1038
Vasal SK, Villegas E, Bjarnason M, Gelaw B, Goertz P (1980) Genetic modifiers and breeding strategies in developing hard endosperm opaque-2 materials. In: Pollmer WG, Phillips RH (eds) Improvement of quality traits of maize for grain and silage use. Nijhoff, London, UK, pp 37–73
Wallace JC, Lopes MA, Paiva E, Larkins BA (1990) New methods for extraction and quantitation of zeins reveal a high content of gamma-zein in modified opaque-2 maize. Plant Physiol 92:191–196
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Lopes, M.A., Takasaki, K., Bostwick, D.E. et al. Identification of two opaque2 modifier loci in Quality Protein Maize. Molec. Gen. Genet. 247, 603–613 (1995). https://doi.org/10.1007/BF00290352
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DOI: https://doi.org/10.1007/BF00290352