Summary
Previous reports have shown that deficient galactosylation of serum IgG occurs in patients with rheumatoid arthritis (RA) and other autoimmune disorders. In this study, we investigated the relationship between galactosylation of serum IgG and disease status in a population of Pima and Papago Indians, who are characterized by a frequent occurrence of RA. The mean galactose levels of serum IgG collected from 11 individuals 1.5–10 years prior to onset (T1), at onset (T2), and 1.7–7 years post-onset (T3) of RA were lower than those from 25 seronegative controls without RA from the same population (P=0.01, 0.005, and ≦0.001, respectively). The mean galactose content of IgG decreased in the order T1>T2>T3, but the differences were not significant. The galactosylation of serum IgG from the RA patients did not differ from that of seropositive Pima Indian controls who did not develop RA over the course of study. The occurrence of IgG galactose deficiency in individuals with RA prior to the onset of the disease supports the contention that this phenomenon may be a risk factor for the development of RA. Furthermore, the occurrence of IgG galactose deficiency in healthy seropositive individuals suggests a relationship between diminished galactosylation and induction of rheumatoid factor (RF).
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References
Mullinax F, Mullinax GL (1975) Abnormality of IgG structure in rheumatoid arthritis and systemic lupus erythematosus (abstract). Arthritis Rheum 18:417–418
Parekh RB, Dwek RA, Sutton BJ, Fernandez DL, Leung A, Stanworth D, Rademacher TW, Mizoguchi T, Taniguchi T, Matsuta K, Takeuchi F, Nagano Y, Miyamoto T, Kobata A (1985) Association of rheumatoid arthritis and primary osteoarthritis with changes in the glycosylation pattern of total serum IgG. Nature 316:452–457
Tomana M, Schrohenloher RE, Koopman WJ, Alarcón GS, Paul WA (1988) Abnormal glycosylation of serum IgG from patients with chronic inflammatory diseases Arthritis Rheum 31:333–338
Parekh R, Isenberg D, Rook G, Roitt I, Dwek R, Rademacher T (1989) A comparative analysis of disease-associated changes in N-glycosylation of serum IgG. J Autoimmun 2:101–114
Tomana M, Schrohenloher RE, Reveille JD, Arnett FC, Koopman WJ (1992) Abnormal galactosylation of serum IgG in patients with systemic lupus erythematosus and members of families with high frequency of autoimmune diseases. Rheumatol Int 12:191–194
Dubé R, Rook GAV, Steele J, Brealey R, Dwek R, Rademacher T, Lennard-Jones J (1990) Agalactosyl IgG in inflammatory bowel disease: correlation with C-reactive protein. Gut 31:431–434
Axford JS, Lydyard PM, Isenberg DA, Mackenzie L, Hay FC, Roit IM (1988) Reduced B cell galactosyltransferase activity in rheumatoid arthritis. Lancet II:1486–1488
Furukawa K, Matsuta K, Takeuchi F, Kosuge E, Miyamoto T, Kobata A (1990) Kinetic study of galactosyltransferase in the B cells of patients with rheumatoid arthritis. Int Immunol 2:105–112
Griffiths HR, Lunec J (1989) The effect of oxygen free radicals on the carbohydrate moiety of IgG. FEBS Lett 245:95–99
del Puente A, Knowler WC, Pettitt DJ, Bennett PH (1988) The incidence of rheumatoid arthritis is predicted by rheumatoid factor titer in a longitudinal population study. Arthritis Rheum 31:1239–1244
Cohen AS (1975) Laboratory diagnostic procedures in the rheumatic diseases. Little, Brown, Boston
Tomana M, Niedermeier W, Spivey C (1978) Microdetermination of monosaccharides in glycoproteins by gas-liquid chromatography. Anal Biochem 89:110–118
Sumar N, Colaco CB, Bodman KB, Parekh R, Williams P, Dwek, Rademacher T, Isenberg DA, Soltys A, Hay FC, Roit IM (1991) Abnormalities in the glycosylation of IgG in spouses of patients with rheumatoid arthritis. J Autoimmun 4:907–914
Parekh RB, Isenberg DA, Roitt IM, Dwek RA, Rademacher TW (1988) Age-related glycosylation of N-linked oligosaccharides of human serum IgG. J Exp Med 167:1731–1736
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Tomana, M., Schrohenloher, R.E., Bennett, P.H. et al. Occurrence of deficient galactosylation of serum IgG prior to the onset of rheumatoid arthritis. Rheumatol Int 13, 217–220 (1994). https://doi.org/10.1007/BF00290198
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DOI: https://doi.org/10.1007/BF00290198