Skip to main content
SpringerLink
Log in

Characterization of some erythrocyte G6PD variants by isoelectric focusing

  • Original Investigations
  • Published:
Human Genetics Aims and scope Submit manuscript

Summary

The existence of a microheterogeneity of glucose-6-phosphate dehydrogenase (G6PD) in human erythrocyte lysates has been previously demonstrated using isoelectric focusing (Der Kaloustian et al., 1974; Turner et al., 1975). The application of this method, modified in some aspects, to the identification of various G6PD variants led to interesting conclusions. The results reported here have been obtained from a study of four distinct molecular types: Gd(-)Mediterranean, Gd(-) Kabyle, the African Gd(+) A, and a new almost undescribed G6PD variant with severe enzyme deficiency named Gd(-) Muret.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Beutler, E., Mathai, L., Smith, J. E.: Biochemical variants of glucose-6-phosphate dehydrogenase giving to congenital non-spherocytic hemolytic disease. Blood 31, 131–149 (1968)

    Google Scholar 

  • Bonsignore, A., Cancedda, R., Lorenzoni, I., Cosulich, M. E., De Flora, A.: Human erythrocyte glucose-6-phosphate dehydrogenase: Physical properties. Biochem. Biophys. Res. Commun. 43, 94–101 (1971)

    Google Scholar 

  • Der Kaloustian, V. M., Idriss-Daouk, S. H., Hallal, R. T., Awdeh, Z. L.: Analysis of human erythrocyte glucose-6-phosphate dehydrogenase isozymes by isoelectric focusing in polyacrylamide gel. Biochem. Genet. 12, 51–58 (1974)

    Google Scholar 

  • Leback, D. H., Wrigley, C. W.: Chromatographic and electrophoretic techniques. In: Zone electrophoresis (I. Smith, ed.), p. 306. London: Heinemann 1976

    Google Scholar 

  • Motulsky, A. G., Yoshida, A.: Methods for the study of red cell glucose-6-phosphate dehydrogenase. In: Biochemical methods in red cell genetics (I. J. Yunis, ed.), p. 51. New York: Academic Press 1969

    Google Scholar 

  • Skala, H., Vibert, M., Kahn, A., Dreyfus, I. C.: Phospho ADP riborylation of human glucose 6 phosphate dehydrogenase: A probable mechanism of the occurrence of hyperanodic forms. Biochem. Biophys. Res. Commun. 89, 988–996 (1979)

    Google Scholar 

  • Turner, B. J., Cederbaum, S. D.: Gel isoelectric focusing and the microheterogeneity of human glucose-6-phosphate dehydrogenase. In: Isozymes. I. Molecular structure (C. Markert, ed.), p. 653. New York: Academic Press 1975

    Google Scholar 

  • Vesterberg, O.: Isoelectric focusing of proteins in polyacrylamide gels. Biochim. Biophys. Acta 257, 11–19 (1972)

    Google Scholar 

  • Wrigley, C. W.: New techniques in amino acid peptide and protein analysis (A. Niederwieser, G. Pataki, eds.), p. 291. Ann Arbor: Science Publish. 1971

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centre d'Hémotypologie du C.N.R.S., CHU de Purpan, F-31052, Toulouse, France

    H. Vergnes & H. Brun

Authors
  1. H. Vergnes
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Brun
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vergnes, H., Brun, H. Characterization of some erythrocyte G6PD variants by isoelectric focusing. Hum Genet 53, 43–45 (1979). https://doi.org/10.1007/BF00289449

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00289449

Keywords

Search

Navigation