Summary
From an F1 hybrid between the two barley (Hordeum vulgare L.) cultivars ‘Golden Promise’ and ‘Mazurka’ a series of doubled haploid (DH) lines were generated both from microspores by anther culture and from immature zygotic embryos after hybridization withH. bulbosum. The DH lines from both sources were used to monitor the segregation of the five major genes, rachilla hair length, DDT susceptibility, height, C hordein polymorphism and mildew resistance. Whereas the microspore-derived samples showed significant departures from the expected 1∶1 ratio for three of the five genes, theH. bulbosum lines showed deviation for only one gene. Analysis of linkage data also showed differences between the two series of DH lines. Cytogenetic analysis revealed a mean chiasma frequency in theH. bulbosum lines which was very similar to the F1 hybrid. In contrast, four of the ten microspore derived lines examined showed a reduced chiasma frequency. One showed evidence of translocation heterozygosity.
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References
Chen CC, Chiu WL, Yu LJ, Ren SS, Yu WJ, Lin MH (1983) Genetic analysis of anther-derived plants of rice: independent assortment of unlinked genes. Can J Genet Cytol 25:18–24
Chen CM, Chen CC, Lin MH (1982) Genetic analysis of anther-derived plants of rice. J Hered 73:49–52
Choo TM, Reinbergs E, Kasha KJ (1985) Use of haploids in breeding barley. Plant Breed Rev 3:219–252
Corduan G (1975) Isozyme variation as an indicator for the generative or somatic origin of anther derived plants ofDigitalis purpurea L. Z Pflanzenzucht 76:47–55
Day A, Ellis THN (1985) Deleted forms of plastid DNA in albino plants from cereal anther culture. Curr Genet 9:671–678
Dunwell JM (1985) Anther and ovary culture. In: Bright SWJ, Jones MGK (eds) Cereal tissue and cell culture. Nijhoff/Junk, Dordrecht, pp 1–44
Edallo S, Zucchianali C, Perenzin M, Salamini F (1981) Chromosomal variation and frequency of spontaneous mutation associated with in vitro culture and plant regeneration in maize. Maydica 26:39–56
Foroughi-Wehr B, Friedt W (1984) Rapid production of recombinant barley yellow mosaic virus resistantHordeum vulgare lines by anther culture. Theor Appl Genet 67:377–382
Hayes JD, Rana MS (1966) Genetic resistance to DDT inHordeum. 1. Linkage studies in diploid barley. Heredity 21:581–593
Huang B, Sunderland N (1982) Temperature-stress pretreatment in barley anther culture. Ann Bot 49:77–88
Huang B, Dunwell JM, Powell W, Hayter AM, Wood W (1984) The relative efficiency of microspore culture and chromosome elimination as methods of haploid production inHordeum vulgare L. Z Pflanzenzucht 92:22–29
Kao KN, Wetter LR, Dyck J, Horn D, Ye CM, Harvey BL (1983) Inheritance of certain genetic traits in pollen plants from F1 hybrid barley. Genet Soc Can Bull 14: D7
Kasha KJ, Reinbergs E (1982) Recent developments in the production and utilization of haploids in barley. In: Proc 4th Int Barley Genet Symp. Edinburgh, pp 655–665
Larkin PJ, Scowcroft WR (1981) Somaclonal variation: a new source of variability from cell cultures for plant improvement. Theor Appl Genet 60:197–214
Lyne LR, Bennett RI, Hunter CP (1986) Embryoid and plant production from cultured barley anthers. In: Alderson PG, Withers LA (eds) Plant Tissue Culture and its Agricultural Applications. Butterworths, Guildford (in press)
Marchylo BA, Kruger JE (1984) Identification of Canadian barley cultivars by reversed-phase high performance liquid chromatography. Cereal Chem 61:295–301
Mather K (1938) The measurement of linkage in heredity. Methuen, London
Nilan RA (1964) The cytology and genetics of barley 1951–1962. Monograph Supplement No 3, Washington State University, Washington
Orton TJ (1979) Haploid barley regenerated from callus cultures ofHordeum vulgare ×H. jubatum. J Hered 71:780–782
Orton TJ, Browers MA (1985) Segregation of genetic markers among plants regenerated from cultured anthers of broccoli (Brassica oleracea var. ‘italica’). Theor Appl Genet 69:637–643
Powell W, Caligari PDS, Dunwell JM (1986) Field performance of lines derived from haploid and diploid tissues ofHordeum vulgare. Theor Appl Genet 72:458–465
Shannon PRM, Nicholson AE, Dunwell JM, Davies DR (1985) Effect of anther orientation on microspore-callus production in barley (Hordeum vulgare L.) Plant Cell Tissue Organ Cult 4:271–280
Shewry PR, Faulks AJ, Pickering RA, Jones IT, Finch RA, Miflin BJ (1980) The genetic analysis of barley storage proteins. Heredity 44:383–389
Sibi M, Biglary M, Demarly Y (1984) Increase in the rate of recombinants in tomato (Lycopersicon esculentum L.) after in vitro regeneration. Theor Appl Genet 68:317–321
Sunderland N, Huang B (1985) Barley anther culture —The switch of programme and albinism. Hereditas (Suppl) 3:27–40
Thomas WTB, Powell W, Wood W (1984) The chromosomal location of the dwarfing gene present in the spring barley variety ‘Golden Promise’. Heredity 53:177–183
Wenzel G, Hoffman F, Thomas E (1976) Heterozygous microspore-derived plants in rye. Theor Appl Genet 48:205–208
Wolfe MS, Schwarzbach E (1978) Patterns of race changes in powdery mildews. Annu Rev Phytopathol 16:159–180
Zamir D, Tanksley SD, Jones RA (1981) Genetic analysis of the origin of plants regenerated from anther tissues ofLycopersicon esculentum Min. Plant Sci Lett 21:223–227
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Powell, W., Borrino, E.M., Allison, M.J. et al. Genetical analysis of microspore derived plants of barley (Hordeum vulgare). Theoret. Appl. Genetics 72, 619–626 (1986). https://doi.org/10.1007/BF00288999
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DOI: https://doi.org/10.1007/BF00288999