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Large double-stranded RNA molecules in Phaseolus vulgaris L. are not associated with cytoplasmic male sterility

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Two large double-stranded RNA molecules, 15 and 16 kilobases, were detected in cytoplasmic male sterile (CMS) Phaseolus vulgaris by agarose gel electrophoresis. A number of smaller RNA molecules were observed in ‘Sprite’, a maintainer line, and recurrent backcrossing of CMS P. vulgarisx‘Sprite’ resulted in a combined electrophoretic pattern of the two large and numerous small RNA molecules. The large RNA molecules were seed and pollen-transmissible, but were not transmitted by grafting. The RNAs were present in revertant and restored lines derived from CMS-Sprite and therefore were not associated with the cytoplasmic male sterile trait.

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Communicated by P. Maliga

Florida Agr. Exp. Stn. Journal Series No. 8405

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Mackenzie, S.A., Pring, D.R. & Bassett, M.J. Large double-stranded RNA molecules in Phaseolus vulgaris L. are not associated with cytoplasmic male sterility. Theoret. Appl. Genetics 76, 59–63 (1988).

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