Summary
Mitochondrial DNA (mtDNA) from fertile (N) and possibly new cytoplasmic male sterile (CMS) genotypes was studied in the sugar beet Beta vulgaris L. It was found by restriction endonuclease analysis that BMC-CMS, a cytoplasm that was derived from the wild beet Beta maritima, contained a unique type of mtDNA which is distinguishable from both the N and S-CMS, the only other CMS genotype that is currently availabe in B. vulgaris L. The organization of three genes: coxI, coxII and cob, was analyzed by hybridization with heterologous probes from maize. These genes have a similar structure in N and BMC-CMS that is different from S-CMS. It is concluded that BMC-CMS is a novel CMS genotype in the sugar beet.
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References
Bosemak NO (1979) Genetic poverty of the sugar beet in Europe. In: Zeven AC, Harten AM van (eds) Proc Conf Broadening Genet Base Crops (1987) Pudoc, Wageningen, pp 29–35
Clark EM, Izhar S, Hanson MR (1985) Independent segregation of the plastid genome and cytoplasmic male sterility in Petunia somatic hybrids. Mol Gen Genet 199:440–445
Coe GE, Stewart D (1977) Cytoplasmic male sterility, self-fertility, and monogermness in Beta maritima L. J Am Soc Sugar Beet Technol 19:257–261
Dawson AJ, Jones VP, Leaver CJ (1984) The apocytochrome b gene in maize mitochondria does not contain introns and is preceded by a potential ribosome binding site. EMBO J 3:2107–2113
Dewey RE, Siedow JN, Timothy DH, Levings III CN (1988) A 13-kilodalton maize mitochondrial protein in E. coli confers sensitivity to Bipolaris maydis toxin. Science 239:293–295
Fluhr R, Aviv D, Edelman M, Galun E (1983) Cybrid containing mixed and sorted-out chloroplasts following interspecific somatic fusions in Nicotiana. Theor Appl Genet 65:289–294
Fox TD, Leaver CJ (1981) The Zea mays mitochondrial gene coding cytochrome oxidase subunit II has an intervening sequence and does not contain TGA codons. Cell 26:315–323
Hallden C, Bryngelsson T, Bosemark NO (1988) Two new types of cytoplasmic male sterility found in wild Beta beets. Theor Appl Genet 75:561–568
Hansen BM, Marcker KA (1984) DNA sequence and transcription of a DNA minicircle isolated from male-fertile sugarbeet mitochondria. Nucleic Acids Res 12:4747–4756
Hooker AL, Smith DR, Lim SM, Beckett JB (1970) Reaction of corn seedlings with male-sterile cytoplasm to Helmintosporium maydis. Plant Dis Rep 54:708–712
Isaac PG, Jones VP, Leaver CJ (1985) The maize cytochrome C oxidase subunit I gene: sequence, expression and rearrangement in cytoplasmic male sterile plants. EMBO J 4:1617–1623
Kinoshita T (1977) Genetic relationship between pollen fertility restoring genes and cytoplasmic factors in the male sterile mutants of sugar beets. Jpn J Breed 27:19–27
Kishima Y, Mikami T, Hirari A, Sugiura M, Kinoshita T (1987) Beta chloroplast genomes: analysis of fraction I protein and chloroplast DNA variation. Theor Appl Genet 73:330–336
Leaver CJ, Hack E, Forde BG (1983) Protein synthesis by isolated plant mitochondria. Methods Enzymol 97:476–484
Lonsdale D (1987) Cytoplasmic male sterility: A molecular perspective. Plant Physiol Biochem 25:256–271
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning; a laboratory manual. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, New York
Mikami T, Sugiura M, Kinoshita T (1984) Molecular heterogeneity in mitochondrial and chloroplast DNAs from normal and male sterile cytoplasms in sugarbeet. Curr Genet 8:319–322
Mikami T, Kishima Y, Sugiura M, Kinoshita T (1985) Organelle genome diversity in sugarbeet with normal and different sources of male sterile cytoplasms. Theor Appl Genet 71:166–171
Mikami T, Harada T, Kinoshita T (1986) Heterogeneity of circular mitochondrial DNA molecules from sugarbeet with normal and male sterile cytoplasms. Curr Genet 10:695–700
Owen FV (1942) Male sterility in sugarbeets produced by complementary effects of cytoplasmic and Mendelian inheritance. Am J Bot (abstr) 29:692
Owen FV (1945) Cytoplasmically inherited male sterility in sugarbeets. J Agric Res 71:423–440
Owen FV (1950) The sugarbeet breeders problem of establishing male sterile populations for hybridization purposes. Proc Am Soc Sugar Beet Technol 6:191–194
Powling A (1981) Species of small DNA molecules found in mitochondria from sugarbeet with normal and male sterile cytoplasms. Mol Gen Genet 183:82–84
Powling A (1982) Restriction endonuclease analysis of mitochondrial DNA from sugarbeet with normal and male sterile cytoplasms. Heredity 49:117–120
Powling A, Ellis THN (1983) Studies on the organelle genomes of sugarbeet with male fertile and male sterile cytoplasms. Theor Appl Genet 65:323–328
Sparks RB, Dale RMK (1980) Characterization of 3H-labelled supercoiled mitochondrial DNA from tobacco suspension culture cells. Mol Gen Genet 180:351–355
Thomas CM (1986) The nucleotide sequence and transcription of minicircular mitochondrial DNAs associated with malefertile and cytoplasmic male-sterile lines of sugarbeet. Nucleic Acids Res 14:9353–9370
Young EG, Hanson MR (1987) A fused mitochondrial gene associated with cytoplasmic male sterility is developmentally regulated. Cell 50:41–49
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Communicated by P. Maliga
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Mann, V., McIntosh, L., Theurer, C. et al. A new cytoplasmic male sterile genotype in the sugar beet Beta vulgaris L.: a molecular analysis. Theoret. Appl. Genetics 78, 293–297 (1989). https://doi.org/10.1007/BF00288814
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DOI: https://doi.org/10.1007/BF00288814