Summary
Electrophoretical analyses of the gliadin fraction extracted from seeds of the intervarietal substitution lines of T. aestivum ssp. spelta in the T. aestivum ssp. vulgare cv ‘Chinese Spring’ for the homoeologous groups 1 and 6 and substitution lines of 6D chromosome of ‘Chinese Spring’ in the durum wheat cv ‘Langdon’ allowed the identification of seeds without gliadin proteins controlled by genes on chromosome 6A and 6B. A gliadin component of ‘Chinese Spring’, not previously assigned to any specific chromosome, is controlled by chromosome 6D in the 6D (6A) and 6D (6B) disomic substitution lines of ‘Langdon’. Additional genes controlling the synthesis of this component may be present on other chromosomes, very likely 6A and 6B, since the analysis of the ‘Chinese Spring’ compensating nullisomic-tetrasomics involving the 6D chromosome does not show the loss of this component or any apparent change in staining intensity. Chromosomal location data and two-dimensional gliadin maps reveal close homologies between the two hexaploid wheats, ‘Chinese Spring’ (T. aestivum ssp. vulgare) and T. aestivum ssp. spelta, belonging to different subspecies in the hexaploid group of genomic formula AABBDD. The comparison of gliadin electrophoretic patterns aiding in the identification of evolutionary pathways in wheat is stressed.
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Brown JWS, Flavell RB (1981) Fractionation of wheat gliadin and glutenin subunits by two-dimensional electrophoresis and the role of group 6 and group 2 chromosomes in gliadin synthesis. Theor Appl Genet 59:349–359
Brown JWS, Law CN, Worland AJ, Flavell RB (1981) Genetic variation in wheat endosperm proteins: an analysis by two-dimensional electrophoresis using intervarietal chromosomal substitution lines. Theor Appl Genet 59:361–371
Cros DL du, Joppa LR, Wrigley CW (1983) Two-dimensional analysis of gliadin proteins associated with quality in durum wheat: chromosomal location of genes for their synthesis. Theor Appl Genet 66:297–302
Galili G, Feldman M (1984) A deficiency of the rapidly migrating high molecular weight glutenin subunit D5 in common wheat. Cereal Res Commun 12:259–261
Johnson BL (1972) Seed protein profiles and the origin of the hexaploid wheats. Am J Bot 59:952–960
Joppa LR, Williams ND (1988) Langdon durum disomic substitution lines and aneuploid analysis in tetraploid wheat. Genome 30:222–228
Joppa LR, Khan K, Williams ND (1983) Chromosomal location of genes for gliadin polypeptides in durum wheat Triticum turgidum L. Theor Appl Genet 64:289–293
Kasarda DD, Lafiandra D, Morris R, Shewry PR (1984) Genetic relationships of wheat gliadin protein. Kulturpflanze 32:41–60
Konarev VG, Gavrilyuk IP, Gubareva NK, Peneva TI (1979) Seed proteins in genome analysis, cultivar identification, and documentation of cereal genetic resources: a review. Cereal Chem 56:272–278
Lafiandra D, Kasarda DD (1985) One and two dimensional (2 pH) polyacrylamide gel electrophoresis in a single gel: separation of wheat proteins. Cereal Chem 62:314–319
Lafiandra D, Benedettelli S, Spagnoletti Zeuli PL, Porceddu E (1983) Genetical aspects of durum wheat gliadins. In: Porceddu E (ed) Breeding methodologies in durum wheat and triticale. Institute of Agricultural Biology, University of Tuscia, Viterbo, pp 29–37
Lafiandra D, Kasarda DD, Morris R (1984) Chromosomal assignment of genes coding for the wheat gliadin protein components of the cultivars Cheyenne and Chinese Spring by two-dimensional electrophoresis. Theor Appl Genet 68:531–539
Lafiandra D, Colaprico G, Kasarda DD, Porceddu E (1987 a) Null alleles for gliadin blocks in bread and durum wheat cultivars. Theor Appl Genet 74:610–616
Lafiandra D, Margiotta B, Porceddu E (1987 b) A possible association between heading time and the Gli-A2 locus in bread wheat. Plant Breed 99:333–335
Payne PI, Holt LM, Hutchinson J, Bennet ND (1984 a) Development and characterization of a line of bread wheat Triticum aestivum, which lacks the short-arm satellite of chromosome 1B and the Gli-B1 locus. Theor Appl Genet 68:327–334
Payne PI, Holt LM, Jackson EA, Law CN (1984 b) Wheat storage proteins: their genetics and their potential for manipulation by plant breeding. Philos Trans R Soc London Ser B 304:359–371
Wrigley CW (1982) The use of genetics in understanding protein composition and grain quality in wheat. Qual Plant-Plant Foods Hum Nutr 31:205–227
Wrigley CW, Shepherd KW (1973) Electrofocusing of grain proteins from wheat genotypes. Ann NY Acad Sci 209:154–162
Wrigley CW, Autran JC, Bushuk W (1982) Identification of cereal varieties by gel electrophoresis of the grain proteins. Adv Cereal Sci 5:211–259
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Lafiandra, D., Benedettelli, S., Margiotta, B. et al. Chromosomal location of gliadin coding genes in T. aestivum ssp. spelta and evidence on the lack of components controlled by Gli-2 loci in wheat aneuploids. Theoret. Appl. Genetics 78, 177–183 (1989). https://doi.org/10.1007/BF00288796
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DOI: https://doi.org/10.1007/BF00288796