Summary
Decapitated seedling root explants of seven cultivars of mothbean (Vigna aconitifolia) cultured on Murashige and Skoog's basal medium without any phytohormone gave rise to plantlets at the end of 4–5 weeks. Addition of cytokinins such as BA, Z, Kn and 2,i-P enhanced the frequency of plant regeneration and also the average number of shoot buds/culture. The buds originated directly from cortical cells or through callussing and subsequent differentiation from the surface. The plantlets obtained were successfully transferred to the field.
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Abbreviations
- BA:
-
benzyladenine
- 2,i-P:
-
6-γ-γ-dimethylallylaminopurine
- Kn:
-
kinetin
- TIBA:
-
2,3,5-Triiodobenzoic acid
- Z:
-
zeatin
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Communicated by G. S. Khush
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Eapen, S., Gill, R. Regeneration of plants from cultured root explants of mothbean (Vigna aconitifolia L. Jacq. Marechal). Theoret. Appl. Genetics 72, 384–387 (1986). https://doi.org/10.1007/BF00288577
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DOI: https://doi.org/10.1007/BF00288577