Summary
Chloroplast (cp) and mitochondrial (mt) DNAs were isolated from four cereal genomes (cultivated wheat, rye, barley and oats) and compared by restriction nuclease analysis. Cleavage of cp and mt DNAs by Sal I, Kpn I, Xho I and EcoR I enzymes indicated that each cereal group contains specific cytoplasmic DNAs. A phylogenetic tree of cereal evolution has been obtained on the basis of cp DNA homologies. It is suggested that wheat and rye diverged after their common ancestor had diverged from the ancestor of barley. This was preceded by the divergence of the common ancestor of wheat, rye and barley and the ancestor of oats.
The molecular weight of the different cp DNAs was determined from the Sal I and Kpn I patterns. cp DNAs from wheat, rye, barley and oats appeared to be characterized by a very similar molecular weight of about 80–82.106 d.
In the case of the mt DNAs, the great number of restriction fragments obtained with the restriction enzymes used prevented precise comparisons and determination of molecular weights.
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Literature
Atchison, B.A.; Whitfeld, P.R.; Bottomley, W. (1976): Comparison of chloroplast DNAs by specific fragmentation with EcoR I endonuclease. Molec. Gen. Genet. 148, 263–269
Avise, J.C.; Giblin-Davidson, C.; Laerm, J.; Patton, J.C.; Lansman, R.A. (1979): Mitochondrial DNA clones and matriarchal phylogeny within and among geographic populations of the pocket gopher, Geomys pinetis. Proc. Natl. Acad. Sci. (Wash.) 76, 6694–6698
Belliard, G.; Vedel, F.; Pelletier, G. (1979): Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion. Nature 281, 401–403
Brown, W.M.; Wright, J.W. (1979): Mitochondrial DNA analyses and the origin and relative age of parthenogenetic lizards. Science 203, 1247–1249
Fiandt, M.; Honigman, A.; Rosenvold, E.C.; Szybalski, W. (1977): Precise measurement of the b2 deletion in coliphage λ. Gene 2, 289–293
Flavell, R.B.; Rimpau, J.; Smith, D.B. (1977): Repeated sequence DNA relationships in four cereal genomes. Chromosoma 63, 205–222
Gingeras, T.R.; Myers, P.A.; Olson, J.A.; Hanberg, F.A.; Roberts, R.J. (1978): A new specific endonuclease present in Xanthomonas holcicola, Xanthomonas papavericola and Brevibacterium luteum. J. Mol. Biol. 118, 113–122
Greene, P.J.; Heynecker, H.L.; Bolivar, F.; Rodriguez, R.L.; Betlach, M.C.; Covarrubias, A.A.; Backman, K.; Russel, D.J.; Tait, R.; Boyer, H.W. (1978): A general method for the purification of restriction enzymes. Nucleic Acids Res. 5, 2373–2380
Hayashi, J.I.; Yonekawa, H.; Gotoh, O.; Tagashira, Y.; Moriwaki, K.; Yosida, T.H. (1979): Evolutionary aspects of variant types of rat mitochondrial DNAs. Biochim. Biophys. Acta 564, 202–211
Herrmann, R.G.; Bohnert, H.J.; Kowallik, K.V.; Schmitt, J.M. (1975): Size, conformation and purity of chloroplast DNA of some higher plants. Biochim. Biophys. Acta 378, 305–317
Kolodner, R.; Tewari, K.K. (1975): the molecular size and conformation of the chloroplast DNA from higher plants. Biochim. Biophys. Acta 402, 372–390
Kung, S.D. (1976): Tobacco fraction 1 protein: a unique genetic marker. Science 191, 429–434
Levings, C.S.; Pring, D.R. (1978): The mitochondrial genome of higher plants. Stadler symp. Univ. of Missouri Columbia, 10, 77–94
Quétier, F.; Vedel, F. (1977): Heterogeneous population of mitochondrial DNA molecules in higher plants. Nature 268, 365–368
Timothy, D.H.; Levings, C.S.; Pring, D.R.; Conde, M.F.; Kermicle, J.L. (1979): Organelle DNA variation and systematic relationships in the genus Zea: Teosinte. Proc. Natl. Acad. Sci. (Wash.) 76, 4220–4224
Vedel, F.; Quétier, F.; Bayen, M. (1976): Specific cleavage of chloroplast DNA from higher plants by EcoR I restriction nuclease. Nature 263, 440–442
Vedel, F.; Quétier, F.; Dosba, F.; Doussinault, G. (1978): Study of wheat phylogeny by EcoR I analysis of chloroplastic and mitochondrial DNAs. Plant Sci. Lett. 13, 97–102
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Communicated by D. von Wettstein
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Vedel, F., Lebacq, P. & Quetier, F. Cytoplasmic DNA variation and relationships in cereal genomes. Theoret. Appl. Genetics 58, 219–224 (1980). https://doi.org/10.1007/BF00288440
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DOI: https://doi.org/10.1007/BF00288440