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Protein A radio-assay of H-Y antigen on human leukocytes using mouse and rat antisera and monoclonal antibodies

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Summary

The presence of H-Y antigen on human leukocytes was investigated using a protein A radio-assay. H-Y antigen could be demonstrated on male cells using either conventional H-Y antisera produced in mice and rats, or monoclonal H-Y antibodies. With mouse antiserum and IgG-type monoclonal antibody the reaction was male-specific using a single antibody. The reaction obtained with rat antiserum was enhanced by the application of a second antibody (rabbit anti-mouse IgG). This technique provides a rapid, simple, objective, and semiquantitative method for the determination of cellular H-Y antigen, the results being expressed as radioactivity bound to the test cells and thus being independent of human observation. It requires only 10–20 ml of blood and small quantities of antiserum or antibody.

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References

  • Adinolfi M, Polani P, Zenthon J (1982) Genetic control of H-Y synthesis. A hypothesis. Hum Genet 61:1–2

    Google Scholar 

  • Böyum A (1968) Separation of leukocytes from blood and bone marrow. Scand J Clin Lab Invest 21; Suppl 97

  • Crichton DN, Steel CM (1980) Radioimmunoassay reveals no antimale specificity in putative H-Y antisera. Clin Exp Immunol 42:597–602

    Google Scholar 

  • Eichwald EJ, Silmser CR (1955) Communication. Transplant Bull 2: 148–149

    Google Scholar 

  • Fellous M, Günther E, Kemler R, Wiels J, Berger R, Guenet JL, Jakob H, Jacob F (1978) Association of the H-Y male-antigen with 194-1 on human lymphoid and differentiated mouse teratocarcinoma cell lines. J Exp Med 148:58–70

    Google Scholar 

  • Goldberg E, Boyse EA, Scheid M, Carswell EA (1971) Serological demonstration of H-Y (male) antigen on mouse sperm. Nature 232: 478–480

    Google Scholar 

  • Goodfellow PN Andrews PW (1982) Sexual differentiation and H-Y antigen. Nature 295:11–13

    Google Scholar 

  • Hall JL, Bushkin Y, Wachtel SS (1981), Immunoprecipitation of human H-Y antigen. Hum Genet 58:34–36

    Google Scholar 

  • Hämmerling U, Stackpole CW Koo GC (1973) Hybrid antibodies for labelling cell surface antigens. In: Methods in cancer research, vol 9. Academic Press, New York, pp 255–282

    Google Scholar 

  • Köhler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256:495–497

    Google Scholar 

  • Koo GC, Stackpole CW, Boyse EA, Hämmerling U, Lardis M (1973) Topographical location of H-Y antigen on mouse spermatozoa by immunoelectron microscopy. Proc Natl Acad Sci USA 70:1502–1505

    Google Scholar 

  • Krco CJ, Goldberg EH (1976) Detection of H-Y (male) antigen on 8-cell mouse embryos. Science 193:1134–1135

    Google Scholar 

  • Müller U (1981) Immunological and functional aspects of H-Y antigen. Hum Genet 58:29–33

    Google Scholar 

  • Ohno S (1976) Major regulatory genes for mammalian sexual development. Cell 7:315–321

    Google Scholar 

  • Ohno S, Epplen JT, Sutou S (1981) Testis-organizing H-Y antigen as a discrete protein; its MHC restricted immune recognition and the genomic environment in which H-Y gene operates. Hum Genet 58: 37–45

    Google Scholar 

  • Savikurki H, Pajunen L, Nemlander A, Andersson LC, de la Chapelle A (1981) Radioimmunoassay for H-Y antigen. J Immunol Methods 41:343–349

    Google Scholar 

  • Scheid M, Boyse EA, Carswell EA, Old LJ (1972) Serologically demonstrable alloantigens of mouse epidermal cells. J Exp Med 135: 938–955

    Google Scholar 

  • Shapiro M, Erickson RP (1981) Evidence that the serological determinant of H-Y antigen is carbohydrate. Nature 290:503–505

    Google Scholar 

  • Silvers WK, Gasser DL, Eicher EM (1982) H-Y antigen, serologically detectable male antigen and sex determination. Cell 28:439–440

    Google Scholar 

  • Wachtel SS, Ohno S, Boyse EA (1975) Possible role for H-Y antigen in the primary determination of sex. Nature 257:235–236

    Google Scholar 

  • Wachtel SS (1983) H-Y antigen and the biology of sex determination, Wachtel SS (ed) Grune and Stratton, New York

    Google Scholar 

  • Wolf U (1979) XY gonadal dysgenesis and the H-Y antigen. Hum Genet 47:269–277

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Medical Genetic, University of Helsinki, Haartmaninkatu, 3, SF-00290, Helsinki 29, Finland

    H. Savikuki & A. de la Chapelle

  2. Department of Pathology, University of Helsinki, SF-00290, Helsinki 29, Finland

    L. C. Andersson

  3. Department of Pediatrics, New York Hospital-Cornell Medical Center, 10021, New York, NY, USA

    S. S. Wachtel

Authors
  1. H. Savikuki
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  2. L. C. Andersson
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  3. S. S. Wachtel
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  4. A. de la Chapelle
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Savikuki, H., Andersson, L.C., Wachtel, S.S. et al. Protein A radio-assay of H-Y antigen on human leukocytes using mouse and rat antisera and monoclonal antibodies. Hum Genet 65, 190–194 (1983). https://doi.org/10.1007/BF00286662

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  • DOI: https://doi.org/10.1007/BF00286662

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