Summary
Since the last report numerous new DBP (Gc) variants have been observed; at present a total of 84 different mutants can be distinguished. Several of them have similar electrophoretic mobilities and/or isoelectric points by conventional isoelectric focusing (IEF). IEF in polyacrylamide gels in the presence of 3 M urea is a convenient and effecient method for the detection of hidden variation.
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References
Aquadro ChF, Avise JC (1981) Genetic diveregence between rodent species assessed by using two dimensional electrophoresis. Proc Natl Acad Sci USA 78:3784–3788
Ayala FJ (1982) Genetic variation in natural populations: problem of electrophoretically cryptic alleles. Proc Natl Acad Sci USA 79: 550–554
Chakraborty R, Nei M (1976) Hidden genetic variability within electromorphs in finite populations. Genetics 84:385–393
Cleve H, Constans J, Berg S, Hoste B, Ishimoto G, Matsumoto H, Spees EK, Weber W (1981) Gc revisited: six further Gc phenotypes delineated by isoelectric focusing and by polyacrylamide gel electrophoresis. Hum Genet 57:312–316
Constans J, Viau M (1977) Group-specific-component: evidence for two subtypes of the Gc1 gene. Science 198:1070–1071
Constans J, Cleve H (1979) Group-specific-component. Report on the first international workshop. Hum Genet 48:143–149
Dykes KK, Copouls B, Polesky H (1983) Description of six new Gc variants. Hum Genet 63:35–37
Papiha SS, Roberts DF, White I, Chahal SMS, Asefi JA (1982) Population genetics of the group-specific-component (Gc) and phosphoglucomutase (PGM1) studied by isoelectric focusing. Am J Phys Anthropol 59:1–7
Salaman MR, Williamson AR (1971) Isoelectric focusing of proteins in the native and denatured states. Biochem J 122:93–99
Spielmann W, Kühnl P (1981) Blutgruppenkunde. G. Thieme, Stuttgart New York
Thymann M, Hjalmarsson K, Svensson M (1982) Five new Gc variants detected by isoelectric focusing in agarose gel. Hum Genet 60:340–343
Weidinger S, Cleve H, Schwarzfischer F, Patutschnik W (1981) The Gc system in paternity examinations; application of Gc subtyping by isoelectrofocusing. In: Hummel K, Gerchow J (eds) Biomathematical evidence of paternity. Springer, Berlin Heidelberg, pp 113–121
Yuasa I, Saneshige Y, Okamoto N, Inoue T, Okada K (1983a) Distribution of Hp, Tf, Gc and Pi polymorphisms in a Japanese population sample from San-In district. J Anthropol Soc Nippon (in press)
Yuasa I, Saneshige Y, Okamoto N, Ikawa S, Hikita T, Ikebuchi J, Inoue T, Okamoto K (1983b) Distribution of Hp. Tf, Gc and Pi polymorphism in a Nepalese population. Hum Hered (in press)
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Constans, J., Cleve, H., Dykes, D. et al. The polymorphism of the vitamin D-binding protein (Gc); Isoelectric focusing in 3 M urea as additional method for identification of genetic variants. Hum Genet 65, 176–180 (1983). https://doi.org/10.1007/BF00286658
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DOI: https://doi.org/10.1007/BF00286658