Summary
Blood samples from four healthy individuals and from seven Fanconi anaemia (FA) patients were cultured at oxygen tensions ranging from 3% to 45% O2. Cultures were harvested at 72 h and scored for chromosomal aberrations. In the majority of FA patients the aberration frequency showed a tendency to increase as a function of oxygen tension over the culture, whereas the aberration frequency in healthy individuals was not affected. However, the response in FA cultures was variable among patients and in individual cases when assayed on different occasions. A much stronger effect of oxygen tension was observed when the FA blood samples had been treated with mitomycin C (0.25 μg/ml, 30 min) before culture initiation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bachur NR, Gee MV, Friedman RD (1982) Nuclear catalyzed antibiotic free radical formation. Cancer Res 42:1078–1081
Balin AK, Goodman DBP, Rasmussen H, Cristofalo VJ (1978) Oxygen-sensitive stages of the cell cycle of human diploid cells. J Cell Biol 78:390–400
Conger AD, Fairchild LM (1952) Breakage of chromosomes by oxygen. Proc Natl Acad Sci (USA) 38:289–299
Dutrillaux B, Aurias A, Dutrillaux AM, Buriot D, Prieur M (1982) The cell cycle of lymphocytes in Fanconi's anaemia. Hum Genet 62:327–332
Fanconi G (1967) Familial constitutional panmyelopathy: Fanconi's anaemia (FA). I. Clinical aspects. Semin Hematol 4:233–240
Fornace AJ Jr, Little JB, Weichselbaum RR (1979) DNA repair in a Fanconi's anaemia fibroblast cell strain. Biochim Biophys Acta 561:99–109
Fujiwara Y, Tatsumi M, Sasaki MS (1977) Cross-link repair in human cells and its possible defect in Fanconi's anemia cells. J Mol Biol 113:635–649
Fujiwara Y (1982) Defective repair of mitomycin C crosslinks in Fanconi's anemia and loss in confluent normal human and xeroderma pigmentosum cells. Biochim Biophys Acta 699: 217–225
Glanz A, Fraser FC (1982). Spectrum of anomalies in Fanconi's anemia. J Med Genet 19:412–416
ISCN (1978) An international system for Human Cytogenetic Nomenclature. Cytogenet Cell Genet 21:309–404
Joenje H, Arwert F, Eriksson AW, De Koning H, Oostra AB (1981) Oxygen dependence of chromosomal aberrations in Fanconi's anaemia. Nature 290:142–143
Kaiser TN, Lojewski A, Dougherty C, Juergens L, Sahar E, Latt SA (1982) Flow cytometric characterization of the response of Fanconi's anemia cells to mitomycin C treatment. Cytometry 2:291–296
Kaye J, Smith C, Hanawalt PC (1980) DNA repair in human cells containing photoadducts of 8-methoxypsoralen or angelicin. Cancer Res 40:696–702
Moorhead PS, Nowell PW, Mellman WJ, Batips DM, Hungerford DA (1960) Chromosome preparations of leukocytes cultured from human peripheral blood. Exp Cell Res 20:613–617
Sasaki MS, Tonomura A (1973) A high susceptibility of Fanconi's anaemia to chromosome breakage by DNA cross-linking agents. Cancer Res 33:1829–1836
Schroeder TM, Anschütz F, Knopp A (1964) Spontane Chromosomenaberrationen bei familiärer Panmyelopathie. Humangenetik 1:194–196
Tomasz M (1976) H2O2 generation during the redox cycle of mitomycin C and DNA-bound mitomycin C. Chem Biol Interactions 13:89–97
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Joenje, H., Oostra, B. Effect of oxygen tension on chromosomal aberrations in Fanconi anaemia. Hum Genet 65, 99–101 (1983). https://doi.org/10.1007/BF00286642
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00286642