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Chemical and genetic analysis of 16S ribosomal RNA in Escherichia coli

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Summary

Comparative chemical analyses of oligonucleotides arising from pancreatic RNase digestions of 16S ribosomal RNAs from Escherichia coli strains K12 and B(H) showed that a decanucleotide fragment, (5Ap,4Gp)Cp, could be detected exclusively in strain K12 but not in strain B(H), in spite of gross similarity of nucleotide distributions between the two strains.

The K12-specific oligonucleotide could not be cotransduced with streptomycin and/or spectinomycin resistant markers from K12 to B(H) by phage Plkc, indicating that the genes specifying 16S ribosomal RNA are not closely linked to these markers on the chromosome.

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Author notes

  1. Akira Muto

    Present address: Institut de Biologie Moléculaire, Université de Genève, 30, Quai de L'École-de-Médecine, Genève

Authors and Affiliations

  1. Research Institute for Nuclear Medicine and Biology, Hiroshima University, Kasumi-cho, Hiroshima, Japan

    Akira Muto, Renkichi Takata & Syozo Osawa

Authors
  1. Akira Muto
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  2. Renkichi Takata
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  3. Syozo Osawa
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Communicated by H. G. Wittmann

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Muto, A., Takata, R. & Osawa, S. Chemical and genetic analysis of 16S ribosomal RNA in Escherichia coli . Molec. Gen. Genet. 111, 15–21 (1971). https://doi.org/10.1007/BF00286550

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  • DOI: https://doi.org/10.1007/BF00286550

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