Abstract
The genome of parsley was studied by DNA/DNA reassociation to reveal its spectrum of DNA reiteration frequencies and sequence organization. The reassociation of 300 nucleotide DNA fragments indicates the presence of four classes of DNA differing in repetition frequency. These classes are: highly repetitive sequences, fast intermediate repetitive sequences, slow intermediate repetitive sequences, and unique sequences. The repeated classes are reiterated on average 136,000, 3000, and 42 times respectively. A minor part of the genome is made up of palindromes. — The organization of DNA sequences in the P. sativum genome was determined by the reassociation kinetics of DNA fragments of varying length. Further information was derived from S1 nuclease resistance and from hyperchromicity measurements on DNA fragments reassociated to defined C0t values. — The portion of the genome organized in a short period interspersion pattern amounts to 47%, with the unique sequences on an average 1000 nucleotides long, and most of the repetitive sequences about 300 nucleotides in length, whereas the weight average length may be up to 600 nucleotides. — About 5% unique DNA and 11% slow intermediate repetitive DNA consist of sequences from 103 up to 104 nucleotides long; these are interspersed with repetitive sequences of unknown length. Long repetitive sequences constitute 33% of the genome, 13% are satellite-like organized, and 20% in long stretches of intermediate repetitive DNA in which highly divergent sequences alternate with sequences that show only minimal divergence. — The results presented indicate remarkable similarities with the genomes of most animal species on which information is available. The most intriguing pecularity of the plant genome derives from its high content of repetitive DNA and the presumed organization of the latter.
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References
Britten, R.J., Graham, D.E., Neufeld, B.R.: An analysis of repeating DNA sequences by reassociation. In: Methods in enzymology (L. Grossman and K. Moldave, eds.), Vol. 29E, pp. 363–418. New York: Academic Press 1974
Britten, R.J., Graham, D.E., Eden, F.C., Painchaud, D.M., Davidson, E.H.: Evolutionary divergence and length of repetitive sequences in sea urchin DNA. J. molec. Evol. 9, 1–23 (1976)
Cairns, J.: The chromosome of E. coli. Cold Spr. Harb. Symp. quant. Biol. 28, 43–46 (1963)
Carroll, D., Brown, D.D.: Repeating units of Xenopus laevis oocyte-type 5S DNA are heterogeneous in length. Cell 7, 467–475 (1976)
Chamberlin, M.E., Britten, R.J., Davidson, E.H.: Sequence organization in Xenopus DNA studied by the electron microscope. J. molec. Biol. 96, 317–333 (1975)
Crain, W.R., Davidson, E.H., Britten, R.J.: Contrasting patterns of DNA sequence arrangement in Apis mellifera (honeybee) and Musca domestica (housefly). Chromosoma (Berl.) 59, 1–12 (1976)
Danna, K.J., Sack, G.H., Nathans, J.D.: Studies of simian virus 40 DNA, VII. A cleavage map of the SV 40 genome. J. molec. Biol. 78, 363–376 (1973)
Darlington, C.D., Wylie, A.P.: Chromosome atlas of flowering plants. London: George Allen 1955
Davidson, E.H., Britten, R.J.: Organization, transcription, and regulation in the animal genome. Quart. Rev. Biol. 48, 555–613 (1973)
Davidson, E.H., Hough, B.R., Amenson, C.S., Britten, R.J.: General interspersion of repetitive with nonrepetitive elements in the DNA of Xenopus. J. molec. Biol. 77, 1–23 (1973)
Davidson, E.H., Klein, W.H., Britten, R.J.: Sequence Organization in animal DNA and a speculation on hnRNA as a coordinate regulatory transcript. Develop. Biol. 55, 69–84 (1977)
Davis, R.W., Simon, M., Davidson, N.: Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. In: Methods in enzymology (L. Grossman and K. Moldave, eds.), Vol. 21 pp. 413–428. New York: Academic Press 1971
Dawid, J.B.: Deoxyribonucleic Acid in amphibian eggs. J. molec. Biol. 12, 581–603 (1965)
Firtel, R.A., Kindle, K.: Structural organization of the genome of the cellular slime mold Dictyostelium discoideum: interspersion of repetitive and single copy DNA. Cell 5, 401–411 (1975)
Flavell, R.B., Bennett, M.D., Smith, J.B., Smith, D.B.: Genome size and the proportion of repeated nucleotide sequence DNA in plants. Biochem. Genet. 12, 257–269 (1974)
Flavell, R.B., Smith, D.B.: Nucleotide sequence organization in the wheat genome. Heredity 37, 231–252 (1976)
Flavell, R.B., Smith, D.B.: Hyperpolymer formation during renaturation of DNA from genomes with different sequence organization. Nucleic Acids Res. 4, 2429–2444 (1977)
Graham, D.E., Neufeld, B.R., Davidson, E.H., Britten, R.J.: Interspersion of repetitive and nonrepetitive DNA sequences in the sea urchin genome. Cell 1, 127–137 (1974)
Hamer, D.H., Thomas, C.A.: Palindrome theory. J. molec. Biol. 84, 139–144 (1974)
Ingle, J., Pearson, G.G., Sinclair, J.: Species distribution and properties of nuclear satellite DNA in higher plants. Nature (Lond.) New Biol. 242, 193–197 (1973)
Kung, S.D.: Expression of chloroplast genomes in higher plants. Ann. Rev. Plant Physiol. 28, 401–437 (1977)
Leuchtenberger, C.: Quantitative determination of DNA in Cells by Feulgen microspectrophotometry. In: General cytochemical Methods (Danielli, J.F., ed.), Vol. 1, pp. 219–278. New York: Academic Press 1958
Mandel, M., Marmur, J.: Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. In: Methods in enzymology (L. Grossman and Moldave, K., eds.), Vol. 12B, pp. 195–200. New York: Academic Press 1968
Manning, J.E., Schmid, C.W., Davidson, N.: Interspersion of repetitive and nonrepetitive DNA sequences in the Drosophila melanogaster genome. Cell 4, 141–155 (1975)
Noll, H.: Characterization of macromolecules by constant velocity sedimentation. Nature (Lond.) 215, 360–363 (1967)
Prunell, A., Bernardi, G.: Fractionation of native and denatured DNA on agarose columns. J. biol. Chem. 248, 3433–3440 (1973)
Rees, H., Jones, R.N.: The origin of the wide species variation in nuclear DNA content. Int. Rev. Cytol. 32, 53–92 (1972)
Rigby, P.W.J., Dieckmann, M., Rhodes, C., Berg, P.: Labeling DNA to high specific activity in vitro by nick translation with DNA polymerase I. J. molec. Biol. 113, 237–251 (1977)
Smith, D.B., Flavell, R.B.: Nucleotide sequence organisation in the rye genome. Biochim. biophys. Acta (Amst.) 474, 82–97 (1977)
Smith, D.B., Rimpau, J., Flavell, R.B.: Interspersion of different repeated sequences in the wheat genome revealed by interspecies DNA/DNA hybridisation. Nucleic Acids Res. 3, 2811–2825 (1976)
Studier, F.W.: Sedimentation studies of the size and shape of DNA. J. molec. Biol. 11, 373–390 (1965)
Walbot, V., Dure, L.S.: Developmental biochemistry of cotton seed embryogenesis and germination. VII. Characterization of the cotton genome. J. molec. Biol. 31, 349–370 (1976)
Wells, R., Royer, H.D., Hollenberg, C.P.: Non Xenopus-like DNA sequence organization in the Chironomus tentans genome. Mol. gen. Genet. 147, 503–509 (1976)
Wetmur, J.G., Davidson, N.: Kinetics of renaturation of DNA. J. molec. Biol. 31, 349–370 (1968)
Wilson, D.A., Thomas, C.A.: Palindromes in chromosomes. J. molec. Biol. 84, 115–144 (1974)
Zimmermann, J.L., Goldberg, R.B.: DNA sequence organization in the genome of Nicotiana tabacum. Chromosoma (Berl.) 59, 227–252 (1977)
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Kiper, M., Herzfeld, F. DNA sequence organization in the genome of Petroselinum sativum (Umbelliferae). Chromosoma 65, 335–351 (1978). https://doi.org/10.1007/BF00286413
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DOI: https://doi.org/10.1007/BF00286413