Abstract
An extracellular glucose-forming amylase was produced by Lactobacillus brevis isolated from ‘Kagasok tea’. The enzyme was purified 70-fold and had optimal activity at 55°C and pH 6.5. Its K m value for starch was 0.27 mg ml-1 and its M r was approx. 75,900 Da. The activity of the enzyme was enhanced by Ca2+, Mg2+, Na+ or K+ and inhibited by EDTA, KCN, citric acid and l-cysteine.
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Ilori, M.O., Amund, O.O. & Omidiji, O. Short communication: Purification and properties of a glucose-forming amylase of Lactobacillus brevis . World J Microbiol Biotechnol 11, 595–596 (1995). https://doi.org/10.1007/BF00286381
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DOI: https://doi.org/10.1007/BF00286381