Abstract
Alkaline phosphatase, excreted by Neurospora crassa preg c and purified to apparent homogeneity by 7.5% PAGE, did not show DNAase activity and removed the terminal 5′-phosphate group from plasmid Bluescript M13+ linearized with EcoRI. The preg c strain may therefore replace other sources of alkaline phosphatase for use in dephosphorylating linearized plasmidial DNA in cloning experiments.
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Pereira, M., Pereira, H., Thedei, G. et al. Purification of Neurospora crassa alkaline phosphatase without DNAse activity for use in molecular biology. World J Microbiol Biotechnol 11, 505–507 (1995). https://doi.org/10.1007/BF00286362
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DOI: https://doi.org/10.1007/BF00286362