Summary
The previously cloned class A β-lactamase gene (bla) of Streptomyces cacaoi was shown to be inducible by β-lactam compounds in the host organism S. lividans. A regulatory region of 2.75 kb was identified and the nucleotide sequence determined. It contained four open reading frames (ORFs) of which only two were complete and required for induction. ORF1-ORF2 exerted a positive regulatory effect on the expression of bla. Inactivation of ORF1 or of ORF2 resulted not only in the loss of induction, but also in a 30- to 60-fold decrease in the basal (non-induced) level of β-lactamase production. ORF1 codes for a DNA-binding protein related to the AmpR repressor/activator, which controls the expression of ampC (class C β-lactamase) genes in several Enterobacteria.
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Lenzini, V.M., Magdalena, J., Fraipont, C. et al. Induction of a Streptomyces cacaoi β-lactamase gene cloned in S. lividans . Molec. Gen. Genet. 235, 41–48 (1992). https://doi.org/10.1007/BF00286179
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DOI: https://doi.org/10.1007/BF00286179