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Mutants partially defective in excision repair at five autosomal loci in Drosophila melanogaster

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Abstract

Primary cell cultures derived from mutants in seventeen different genes were analyzed for their ability to excise pyrimidine dimers from DNA. Five of these mutagen-sensitive mutants [mus(2)205A1, mus(3)302D1, mus(3) 304D3, mus(3)306D1, mus(3)308D2] display a significantly reduced excision capacity relative to control cultures. In addition, two of the five [mus(3)306D1, mus(3)308D2] are defective in the accumulation of single-strand breaks normally seen after ultraviolet irradiation. This study, therefore, brings the total number of Drosophila mutants known to be defective in excision repair to seven. The results are discussed relative to other genetic and biochemical properties of these mutants.

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Abbreviations

UV:

ultraviolet irradiation

MMS:

methyl methanesulfonate

HN2:

nitrogen mustard (mechlorethamine)

References

  • Baker, B.S., Boyd, J.B., Carpenter, A.T.C., Green, M.M., Nguyen, T.D., Ripoll, P., Smith, P.D.: Genetic controls of meiotic recombination and somatic DNA metabolism in Drosophila melanogaster. Proc. nat. Acad. Sci. (Wash.) 73, 4140–4144 (1976)

    CAS  Google Scholar 

  • Beukers, R., Berends, W.: Isolation and identification of the irradiation product of thymine. Biochim. Biophys. Acta (Amst.) 41, 550–551 (1960)

    Article  CAS  Google Scholar 

  • Boyce, R.P., Howard-Flanders, P.: Release of ultraviolet light-induced thymine dimers from DNA in E. coli K-12. Proc. nat. Acad. Sci. (Wash.) 51, 293–300 (1964)

    CAS  Google Scholar 

  • Boyd, J.B., Golino, M.D., Setlow, R.B.: The mei-9a mutant of Drosophila melanogaster increases mutagen sensitivity and decreases excision repair. Genetics 84, 527–544 (1976)

    CAS  PubMed  Google Scholar 

  • Boyd, J.B., Harris, P.V., Osgood, C.J., Smith, K.E.: Biochemical characterization of repair-deficient mutants of Drosophila. In: DNA repair and mutagenesis in eukaryotes (W.M. Generoso, M.D. Shelby and F.J. de Serres, eds.), pp. 209–221. New York: Plenum Press 1980

    Google Scholar 

  • Boyd, J.B., Setlow, R.B.: Characterization of postreplication repair in mutagen-sensitive strains of Drosophila melanogaster. Genetics 84, 507–526 (1976)

    CAS  PubMed  Google Scholar 

  • Buhl, S.N., Setlow, R.B., Regan, J.D.: DNA repair in Potorous tridactylus. Biophys. J. 14, 791–803 (1974)

    CAS  PubMed  Google Scholar 

  • Cleaver, J.E.: Defective repair replication of DNA in xeroderma pigmentosum. Nature (Lond.) 218, 652–656 (1968)

    CAS  Google Scholar 

  • Dunn, W.C., Regan, J.D.: Inhibition of DNA excision repair in human cells by arabinofuranosyl cytosine: Effect on normal and xeroderma pigmentosum cells. Molec. Pharmacol. 15, 367–374 (1979)

    CAS  Google Scholar 

  • Hanawalt, P.C., Cooper, P.K., Ganesan, A.K., Smith, C.A.: DNA repair in bacteria and mammalian cells. Ann. Rev. Biochem. 48, 783–836 (1979)

    CAS  PubMed  Google Scholar 

  • Harris, P.V., Boyd, J.B.: Excision repair in Drosophila. Analysis of strand breaks appearing in DNA of mei-9 mutants following mutagen treatment. Biochim. biophys. Acta (Amst.) 610, 116–129 1981)

    Google Scholar 

  • Keijzer, W., Jaspers, N.G.J., Abrahams, P.J., Taylor, A.M.R., Arlett, C.F., Zelle, B., Takebe, H., Kinmont, P.D.S., Bootsma, D.: A seventh complementation group in excision-deficient xeroderma pigmentosum. Mutation Res. 62, 183–190 (1979)

    CAS  PubMed  Google Scholar 

  • Kohn, K.W., Erickson, L.C., Ewig, R.A.G., Friedman, C.A.: Fractionation of DNA from mammalian cells by alkaline elution. Biochemistry 15, 4629–4637 (1976)

    Article  CAS  PubMed  Google Scholar 

  • Lehmann, A.R., Kirk-Bell, S., Arlett, C.F., Harcourt, S.A., de Weerd-Kastelein, E.A., Keijzer, W., Hall-Smith, P.: Repair of ultraviolet light damage in a variety of human fibroblast cell strains. Cancer Res. 37, 904–910 (1977)

    CAS  PubMed  Google Scholar 

  • Lindsley, D.L., Grell, E.H.: Genetic variations of Drosophila melanogaster. Carnegie Inst. Washington Publ. 647, 1980

  • Pettijohn, D., Hanawalt, P.: Evidence for repair replication of ultraviolet damaged DNA in bacteria. J. molec. Biol. 9, 395–410 (1964)

    CAS  Google Scholar 

  • Reynolds, R.J.: Removal of pyrimidine dimers from Saccharomyces cerevisiae nuclear DNA under nongrowth conditions as detected by a sensitive enzymatic assay. Mutation Res. 50, 43–56 (1978)

    CAS  PubMed  Google Scholar 

  • Setlow, R.B., Carrier, W.L.: The disappearance of thymine dimers from DNA: An error-correcting mechanism. Proc. nat. Acad. Sci. (Wash.) 51, 226–231 (1964)

    CAS  Google Scholar 

  • Smith, P.D., Snyder, R.D., Dusenberg, R.L.: Isolation and characterization of repair-deficient mutants of Drosophila melanogaster. In: DNA repair and mutagenesis in eukaryotes (W.M. Generoso, M.D. Shelby, F.J. de Serres, eds.), pp. 175–188. New York: Plenum Press 1980

    Google Scholar 

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Authors and Affiliations

  1. Department of Genetics, University of California, 95616, Davis, California, USA

    James B. Boyd & Paul V. Harris

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  1. James B. Boyd
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  2. Paul V. Harris
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This work is dedicated to Professor W. Beermann whose own contributions were instrumental in focusing a modern analysis of the eukaryotic genome on the diptera. Those of us who benefitted so much from his personal guidance recognize that we did so as a result of some sacrifice on his part. One of Boyd's contemporaries in Tübingen once remarked: “It's terrifying to think what Professor Beermann could do if he were in the lab full time.”

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Boyd, J.B., Harris, P.V. Mutants partially defective in excision repair at five autosomal loci in Drosophila melanogaster . Chromosoma 82, 249–257 (1981). https://doi.org/10.1007/BF00286109

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  • DOI: https://doi.org/10.1007/BF00286109

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