Abstract
Fragments from section 3 of the salivary gland X chromosome of D. melanogaster were dissected with a micromanipulator. The DNA was extracted, cut and ligated to a λ vector in a volume of a few nanoliters in an oil chamber monitored through a microscope. From about 10 pg of DNA we obtained 80 recombinant clones, a sample of which were analysed and shown to contain Drosophila DNA which hybridises in situ to the region of section 3 of the X chromosome. With this technique we can isolate clones from any desired region as small as 200 kb from the euchromatic arms of polytene chromosomes.
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This paper is dedicated to Professor W. Beermann on the occasion of his sixtieth birthday
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Scalenghe, F., Turco, E., Edström, J.E. et al. Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes. Chromosoma 82, 205–216 (1981). https://doi.org/10.1007/BF00286105
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DOI: https://doi.org/10.1007/BF00286105