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Microfluorometric studies on chromosomes

Quantitative determination of protein content of Chinese hamster chromosome 1 in situ with and without trypsin digestion

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Abstract

The fluorescent stains, dansyl chloride and fluorescamine, were used to indicate the amount of protein in G-banded and unbanded chromosomes 1 of the Chinese hamster relative to the average amount of protein in human erythrocyte. Fluorescence was found to be proportional to protein mass up to the equivalent of three erythrocytes. In G-banding, trypsin digestion resulted in an average protein loss of 35.4% compared with unbanded chromosomes.

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Authors and Affiliations

  1. Division of Morbid Anatomy, Department of Pathology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong

    Joseph C. K. Lee

  2. Department of Cellular Pathology, Armed Forces Institute of Pathology, 20306, Washington, DC, USA

    G. F. Bahr

Authors
  1. Joseph C. K. Lee
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  2. G. F. Bahr
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Lee, J.C.K., Bahr, G.F. Microfluorometric studies on chromosomes. Chromosoma 88, 374–376 (1983). https://doi.org/10.1007/BF00285859

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  • DOI: https://doi.org/10.1007/BF00285859

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