Abstract
The region 9E1-2 — 10B1-2 of the Drosophila melanogaster X chromosome was analysed under the light (LM) and the electron (EM) microscope using different fixatives and an EM map of the region was constructed. EM analysis revealed 21 bands in the region 9E1-2 — 10B1-2 instead of 36 bands in Bridges' map. This descrepancy mainly results from the fact that 14 bands indicated as “doublets” by Bridges appear as a single bands. No doublets were found in the whole 9B1-2 — 10C1-2 region after fixation of salivary glands in 3% glutaraldehyde, 3% formaldehyde and 3∶1 ethanol-acetic acid mixture. 45% acetic acid is the only fixative which results in strongly vacuolated appearance of the bands. — The break points of 30 chromosome rearrangements in the region 9E1-2 — 10B1-2 were located under EM or LM within the limits of the EM map of this region.
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Akifjev, A.P.: “Siling” DNA and its role in evolution. Priroda (Moscow) 709, 49–55 (1977)
Alikhanian, S.I.: A study of the lethal mutations in the left end of the sex chromosome in Drosophila melanogaster. Zool. Zhurn. (Moscow) 16, 247–279 (1937)
Ashburner, M.: Some aspects of the structure and function of the polytene chromosomes of the Diptera. In: Insect cytogenetics (R. Blackman, G. Hewitt and M. Ashburner, eds). Oxford: Blackwell Scientific Publ., In press, 1980
Atherton, D., Gall, J.: Salivary gland squashes for in situ nuclei acid hybridization studies. Dros. Inf. Serv. 49, 131–133 (1972)
Barrett, J.A.: The estimation of the number of mutationally silent loci in saturation mapping experiments. Genet. Res. (Camb.) 35, 33–44 (1980)
Beermann, W.: Chromosomes and genes. In: Results and problems in cell differentiation, (Beermann, W. ed.) vol. pp. 1–33. 4, Berlin-Heidelberg-New York: Springer 1972
Berendes, H.D.: Polytene chromosome structure at the submicroscopic level. I. A map of region X, 1-4E of Drosophila melanogaster. Chromosoma (Berl.) 29, 118–130 (1970)
Bonner, J., Wu, J.-R.: A proposal for the structure of the Drosophila genome. Proc. nat. Acad. Sci (Wash.) 70, 535–537 (1973)
Bridges, C.B.: A revised map of the salivary gland X-chromosome of Drosophila melanogaster. J. Hered. 29, 11–13 (1938)
Britten, R.J., Davidson, E.: Organization, transcription and regulation in the animal genome. Quart. Rev. Biol. 48, 565–613 (1973)
Callan, H.G.: Functional units. Introductionary remarks. Phil. Trans, roy. Soc. B 283, 381–382 (1978)
Craymer, L., Roy, E.: Report on Drosophila melanogaster new mutants Dros. Inf. Serv. 55, 200–204 (1980)
Crick, F.H.C.: General model for the chromosomes of higher organisms. Nature (Lond.) 234, 25–27 (1971)
Dickinson, W.J., Sullivan, D.T.: Gene-enzyme systems in Drosophila. Berlin-Heidelberg-New York: Springer 1975
Gersh, E.S.: Sites of gene activity and of inactive genes in polytene chromosomes of Diptera. J. theor. Biol. 50, 413–428 (1975)
Jamrich, M., Greenleaf, A.L., Bautz, E.K.F.: Localization of RNA polymerase in Drosophila melanogaster polytene chromosomes. Proc. nat. Acad. Sci (Wash.) 74, 2079–2083 (1977)
Johnson, M.M., Woloshyn, E.P., Nash, D.: Cytogenetic localisation of the purinel and guanosine 1 loci of Drosophila melanogaster; the purine 1 locus specifies a vital function. Molec. gen. Genet. 174, 287–292 (1979)
Judd, B.H., Shen, M.W., Kaufman, T.C.: The anatomy and function of a segment of the X chromosome of Drosophila melanogaster. Genetics 71, 139–156 (1972)
Keppy, D.O., Welshons W.J.: The synthesis of compound bands in Drosophila melanogaster salivary gland chromosomes. Chromosoma (Berl.) 76, 191–200 (1980)
Kiknadze I.I.: Functional organization of chromosomes. In: Uspehi Sovremennoy Genetiki, (N.P. Dubinin, ed.), 175–205 Moscow, Nauka; 1971
Lefevre, G., Jr.: The eccentricity of vermilion deficiencies of Drosophila melanogaster. Genetics 63, 589–600 (1969)
Lefevre, G., Jr.: Salivary chromosome bands and the frequency of crossing over in Drosophila melanogaster. Genetics 67, 497–513 (1971)
Lefevre, G., Jr.: The one band-one gene hypothesis: evidence from a cytogenetic analysis of mutant and non-mutant rearrangement breakpoints in Drosophila melanogaster. Cold Spr. Harb. Symp. quant. Biol. 38, 591–599 (1974)
Lefevre, G., Jr.: A photographic representation and interpretation of the polytene chromosomes of Drosophila melanogaster salivary glands. In: The genetics and biology of Drosophila, (M. Ashburner and E. Novitsky, eds.), vol. 1a, pp. 1–66. London, New York, San Francisco: Academic Press 1976
Lefevre, G., Jr., Wiedenheft, K.B.: Two genes in one band? Dros. Inf. Serv. 51, 83 (1974)
Lindsley, D.L., Grell, E.H.: Genetic variations of Drosophila melanogaster. Carnegie Inst. Washington, Publ. No. 627, 1968
Olenov, Yu. M.: On the genome organization in Metazoa. Tsitologia (Leningrad) 16, 403–419 (1974)
Paul, J.: General theory of chromosomes structure and gene activation in eukaryotes. Nature (Lond.) 238, 444–446 (1972)
Pelling, C.: A replicate and synthetic chromosomal unit — the modern concept of the chromomere. Proc. roy. Soc. B 164 279–289 (1966)
Semeshin, V.F., Zhimulev, I.F., Belyaeva, E.S.: Electron microscope autoradiographic study on transcriptional activity of Drosophila melanogaster polytene chromosomes. Chromosoma (Berl.) 73, 163–177 (1979a)
Semeshin, V.F., Zhimulev, I.F., Belyaeva, E.S.: Cytogenetic study of the region 9E-10A of Drosophila melanogaster X chromosome. I. Morphology of the region and mapping of deletions affecting the band 10A1-2. Genetica (U.S.S.R.) 15, 1784–1792 (1979b)
Skaer, B.J.: Interband transcription in Drosophila. J. Cell Sci. 26, 251–266 (1977)
Sorsa, V.: A cytological view of the functional organization of chromomeres. Hereditas (Lund) 82, 63–68 (1976)
Sorsa, V., Saura, A.O.: Electron microscopic analysis of the banding pattern in the salivary gland chromosomes of Drosophila melanogaster. Divisions 3, 4 and 5 of X. Hereditas (Lund) 92. 341–351 (1980)
Speiser, Ch.: Eine Hypothese über die funktionelle Organization der Chromosomen höherer Organismen. Theor. Appl. Genet. 44, 97–99 (1974)
Stewart, B., Meriam, J.R.: Segmental aneuploidy of the X chromosome. Dros. Inf. Serv. 50, 167–170 (1973)
Woodruff, R.C., Ashburner, M.: The genetics of a small autosomal region of Drosophila melanogaster containing the structural gene for alcohol dehydrogenase. I. Characterization of deficiencies and mapping of Adh and visible mutations. Genetics 92, 117–132 (1979)
Young, M.W., Judd, B.H.: Non-essential sequences, genes and the polytene chromosome bands of Drosophila melanogaster. Genetics 88, 723–742 (1978)
Zhimulev, I.F., Belyaeva, E.S., Semeshin, V.F.: Informational content of polytene chromosome bands and puffs. In: Critical reviews in biochemistry. Boca. Raton Florida: CRC Press. Inc. (in press, 1981a)
Zhimulev, I.F., Pokholkova, G.V., Bgatov, A.V., Semeshin, V.F., Belyaeva, E.S.: Fine cytogenetical analysis of the 10A1-2 band and the adjoining regions in the Drosophila melanogaster X chromosome. II. Genetical analysis. Chromosoma (Berl.) 82, 25–40 (1981b)
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Zhimulev, I.F., Semeshin, V.F. & Belyaeva, E.S. Fine cytogenetical analysis of the band 10a1-2 and the adjoining regions in the Drosophila melanogaster X chromosome. Chromosoma 82, 9–23 (1981). https://doi.org/10.1007/BF00285746
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DOI: https://doi.org/10.1007/BF00285746