Abstract
A 5.6 kb DNA fragment from the fungus Phycomyces blakesleeanus has been cloned and sequenced. The fragment contains a gene that probably codes for the enzyme acetyl-coenzyme A synthetase (facA). The amino acid sequence deduced for the P. blakesleeanns protein is highly homologous to those of acetyl-coA-synthetases from other organisms. When placed under the control of a constitutive promoter from Aspergillus nidulans, the cloned gene complemented a facA mutation of this organism. In P. blakesleeanns, the expression of facA is induced by acetate.
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Communicated by C. van den Hondel
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Garre, V., Murillo, F.J. & Torres-Martínez, S. Isolation of the facA (acetyl-CoA synthetase) gene of Phycomyces blakesleeanus . Molec. Gen. Genet. 244, 278–286 (1994). https://doi.org/10.1007/BF00285455
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DOI: https://doi.org/10.1007/BF00285455