Summary
Annulate lamellae were observed in untreated in vivo ascites tumor cells with a diminshed cytoplasmic microtubule complex. The ascites tumor cells in vitro responded to prolonged colchicine treatment with the formation of annulate lamellae. Simultaneous treatment with dibutyryl cyclic adenosine monophosphate and colchicine seemed to enhance the formation of annulate lamellae. Single pore complexes were found in the granular endoplasmic reticulum in untreated tumor cells in vitro, and a close association of microtubules with the nuclear envelope was observed. Our results suggest the existence of an interrelationship between the cytoplasmic microtubule complex and certain other cell structures, i.e. the nuclear envelope, annulate lamellae, and single pore complexes.
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Bichel,P., Barfod,N.M., Jakobsen,A.: Employment of synchronized cells and flow microfluorometry in investigations on the JB-1 ascites tumor chalones. Virchows Arch. B Cell Path. 19, 127–133 (1975)
Borman,L.S., Dumont,J.N., Hsie,A.W.: Relationship between cyclic AMP, microtubule organization, and mammalian cell shape. Studies on Chinese hamster ovary cells and their variants. Exp. Cell. Res. 91, 422–428 (1975)
Brinkley,B.R., Fuller,G.M., Highfield,D.P.: Tubulin antibodies as probes for microtubules in dividing and nondividing mammalian cells. In: Cell motility. Book A. Motility, muscle and non-muscle cells, Vol. 3, eds. R. Goldman, T. Pollard, and I. Rosenbaum. Cold Spring Harbor Laboratory 1976
Chambers,V.C., Weiser,R.S.: Annulate lamellae in Sarcoma I cells J. Cell Biol. 21, 133–139 (1964)
Chemnitz,J., Skaaring,P., Bichel,P.: Light and electron microscopy of the JB-1 ascites tumor at different stages of growth. Z. Krebsforsch. 82, 111–131 (1974)
Chemnitz,J., Salmberg,K., Bierring,F.: Observations on the association of annulate lamellae with vinblastine-induced paracrystals in tumor cells in vitro. Virchows Arch. B. Cell Path. 24, 147–156 (1977)
De Brabander,M., Borgers,M.: The formation of annulate lamellae induced by the disintegration of microtubules. J. Cell Sci. 19, 331–340 (1975)
Dombernowsky,P., Bichel,P., Hartmann,N.R.: Cytokinetic analysis of the JB-1 ascites tumor at different stages of growth. Cell Tiss. Kinet. 6, 347–357 (1973)
Goldstein,M.N.: Annulate lamellae in cultured human neuroblastoma cells. Cancer Res. 31, 209–213 (1971)
Hagon,E.E., Gunz,F.W.: Annulate lamellae in human lymphosarcoma and chronic lymphocytic leukaemia. Cytobios 7, 7–14 (1973)
Hinek,A., Thyberg,J., Friberg,U.: Electron microscopic studies on embryonic chick spinal ganglion cells: relationship between microtubules and the Golgi complex. J. Neurocytol. 6, 13–25 (1977)
Karnovsky,M.J.: A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 27, 137a (1965)
Kessel,R.G.: Annulate lamellae. J. Ultrastruct. Res. Suppl. 10, 1–82 (1968)
Locker,J., Goldblatt,P.J., Leighton,J.: Hematogenous metastasis of Yoshida ascites hepatoma in the chick embryo liver: ultrastructural changes in tumor cells. Cancer Res. 29, 1244–1253 (1969)
Maul,G.G.: Annulate lamellae and single pore complexes in normal, SV 40-transformed and tumor cells in vitro. A semiquantitative analysis. Exp. Cell Res. 104, 233–245 (1977)
McCulloch,D.: Fibrous structure in the ground cytoplasm of the Arbacia egg. J. exp. Zool. 119, 47–63 (1952)
Pickett-Heaps,J.: The evolution of mitosis and the eukaryotic condition. Biosystems 6, 37–48 (1974)
Porter,K.R., Puck,T.T., Hsie,A.W., Kelley,D.: An electron microscope study of the effects of dibutyryl cyclic AMP on Chinese hamster ovary cells. Cell 2, 145–162 (1974)
Roberts,D.K., Marshall,R.B., Wharton,J.T.: Ultrastructure of ovarian tumors. I. Papillary serous cystadenocarcinoma. Cancer 25, 947–958 (1970)
Stadler,J., Franke,W.W.: Colchicine-binding proteins in chromatin and membranes. Nature (New Biol.) 237, 237–238 (1972)
Watanabe,S., Berard,C.W., Triche,T.: Annulate lamellae in four cases of diffuse lymphocytic lymphoma. J. Nat. Cancer Inst. 58, 777–780 (1977)
Wessel,W., Bernhard,W.: Vergleichende electronenmikroskopische Untersuchung von Ehrlich- und Yoshida-Asckestumorzellen. Z. Krebsforsch. 62, 140–162 (1957)
Wischnitzer,S.: The annulate lamellae. Int. Rev. Cytol. 27, 65–100 (1970)
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Chemnitz, J., Salmberg, K. Interrelationship between annulate lamellae and the cytoplasmic microtubule complex in tumor cells in vivo and in vitro. Z. Krebsforsch. 90, 175–185 (1977). https://doi.org/10.1007/BF00285324
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DOI: https://doi.org/10.1007/BF00285324