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The use of graft copolymers as enzyme supports

The preparation and use of polyethylene-co-acrylic acid supports

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Summary

A study has been made of the radiation-induced grafting of acrylic acid onto polyethylene. Grafting was found to be steady state in character in the early stages, when carried out in a suitable medium. Evidence is presented showing that, in the later stages, autoacceleration becomes a feature of the grafting process. Thermal analysisprocedures were used in an attempt to show the occurrence of grafting.

Immobilization using N-ethoxycarbonyl-2-ethoxy,-1,2-dihydroquinoline (EEDQ) as the coupling agent was attempted. This met with various levels of success. The copolymer-EEDQ-enzyme system was used to couple bovine serum albumin (65 mg protein/g of substrate) and phenyl propylamine (95 mg phenylpropylamine/g of substrate).

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Authors and Affiliations

  1. School of Health and Applied Sciences, Leeds Polytechnic, Calverley Street, LS1 3HE, Leeds, UK

    C. G. Beddows

  2. The Department of Colour Chemistry, University of Leeds, LS2 9JT, Leeds, UK

    H. Gil & J. T. Guthrie

Authors
  1. C. G. Beddows
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  2. H. Gil
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  3. J. T. Guthrie
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Beddows, C.G., Gil, H. & Guthrie, J.T. The use of graft copolymers as enzyme supports. Polymer Bulletin 3, 645–653 (1980). https://doi.org/10.1007/BF00285301

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  • DOI: https://doi.org/10.1007/BF00285301

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