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Satellite DNA associated with heterochromatin in Rhynchosciara

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Abstract

The DNA of Rhynchosciara hollaenderi was examined using isopycnic centrifugation in neutral CsCl. Two low density minor bands (collectively termed satellite DNA) were detected in addition to the main band DNA. Main band DNA has a buoyant density of 1.695 g/cm3. The larger of the two minor bands has a buoyant density of 1.680 g/cm3 while the smaller of the two minor bands has a buoyant density of about 1.675 g/cm3. Thermal denaturation studies have confirmed the presence of the two minor classes of DNA.—The satellite and main band DNAs were isolated in relatively pure form and were transcribed in vitro using DNA-dependent RNA polymerase from Escherichia coli. Annealing of the two complementary RNAs (cRNAs) with main band and satellite DNA was examined using filter hybridization techniques.—The chromosomal distribution of the satellite DNA was determined by in situ molecular hybridization of satellite-cRNA with Rhynchosciara salivary gland chromosomes. Satellite-cRNA hybridized with the centromeric heterochromatin of each of the four chromosomes (A, B, C, and X) and with certain densely staining bands in the telomere regions of the A and C chromosomes. Main band-cRNA annealed with many loci scattered throughout the chromosomes including areas containing satellite DNA.

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  1. Department of Biology, Yale University, New Haven, Connecticut

    Ronald A. Eckhardt & Joseph G. Gall

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  1. Ronald A. Eckhardt
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  2. Joseph G. Gall
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Eckhardt, R.A., Gall, J.G. Satellite DNA associated with heterochromatin in Rhynchosciara . Chromosoma 32, 407–427 (1971). https://doi.org/10.1007/BF00285252

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  • DOI: https://doi.org/10.1007/BF00285252

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