Abstract
Incubation of Chironomus salivary glands with α-amanitine in concentrations from 1 to 10 γ/ml results in the suppression of puffing and chromosomal 3H-uridine incorporation after 30 to 60 min in 80% of the cells. Nucleolar 3H-uridine incorporation remains completely unaffected. Even 4 h after the injection of high doses of α-amanitine into living larvae, nucleolar incorporation is still pronounced. The distribution of “resistant” cells within the salivary glands suggests that the uptake of α-amanitine is subject to physiological restrictions.—A puff typically induced during in vitro incubation of salivary glands was found to be less sensitive to α-amanitine than the Balbiani rings.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Beermann, W.: Control of differentiation at the chromosomal level. J. exp. Zool. 157, 49–62 (1964).
—: Differentiation at the level of the chromosomes. In: Cell differentiation and morphogenesis, pp. 24–54. Amsterdam: North-Holland Publ. Co. 1966.
Clever, U.: Genaktivitäten in den Riesenchromosomen von Chironomus tentans und ihre Beziehungen zur Entwicklung. II. Das Verhalten der Puffs während des letzten Larvenstadiums und der Puppenhäutung. Chromosoma (Berl.) 13, 385–436 (1962).
Fiume, L., Stirpe, F.: Decreased RNA content in mouse liver nuclei after intoxication with α-amanitine. Biochim. biophys. Acta (Amst.) 123, 643–645 (1966).
Jacob, S. T., Sajdel, B. M., Munro, H. N.: Different responses of soluble whole nuclear RNA polymerase and soluble nucleolar RNA polymerase to divalent cations and to inhibition by α-amanitine. Biochem. biophys. Res. Commun. 38, 765–770 (1970).
Kedinger, C., Gniazdowski, M., Mandel, J. L., Jr., Gissinger, F., Chambon, P.: α-Amanitine: a specific inhibitor of two DNA-dependent RNA-polymerase activities from calf thymus. Biochem. Biophys. Res. Commun. 38, 165–171 (1970).
Lindell, T. J., Weinberg, F., Morris, P. W., Roeder, R. G., Rutter, W. J.: Specific inhibition of nuclear RNA polymerase II by α-amanitine. Science 170, 447–449 (1970).
Loewenstein, W. R.: Permeability of membrane junctions. Ann. N.Y. Acad. Sci. 137, 441–472 (1966).
Mancino, G., Nardi, I., Corvaja, N., Fiume, L., Marinozzi, V.: Effects of α-amanitin on Triturus lampbrush chromosomes. Exp. Cell Res. 64, 237–239 (1970).
Maul, G. G., Hamilton, T. H.: The intra-nuclear localization of two DNA-dependent RNA polymerase activities. Proc. nat. Acad. Sci. (Wash.) 57, 1371–1378 (1967).
Pelling, C.: Ribonukleinsäure-Synthese der Riesenchromosomen. Autoradiographische Untersuchungen an Chironomus tentans. Chromosoma (Berl.) 15, 71–122 (1964).
Pogo, A. O., Littau, V. C., Allfrey, V. G., Mirsky, A. E.: Modification of ribonucleic acid synthesis in nuclei isolated from normal and regenerating liver: some effects of salt and specific divalent cations. Proc. nat. Acad. Sci. (Wash.) 57, 743–750 (1967).
Politoff, A., Socolar, S. J., Loewenstein, W. R.: Metabolism and the permeability of cell membrane junctions. Biochim. biophys. Acta (Amst.) 135, 791–793 (1967).
Stirpe, F., Fiume, L.: Studies on the pathogenesis of liver necrosis by α-amanitin. Effect of α-amanitin on ribonucleic acid synthesis and on ribonucleic acid polymerase in mouse liver nuclei. Biochem. J. 105, 779–782 (1967).
Wieland, T.: Chemical and toxicological studies with cyclopeptides of Amanita phalloides. Pure Appl. Chem. 6, 339 (1963).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Beermann, W. Effect of α-amanitine on puffing and intranuclear RNA synthesis in Chironomus salivary glands. Chromosoma 34, 152–167 (1971). https://doi.org/10.1007/BF00285183
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00285183