Abstract
Various modifications of reported banding techniques were performed using several cultivars of the genus Tulipa. Banding was obtained with Giemsa using a modified BSG technique and is reported for three cultivars. The chromosome banding noted in all cultivars was confined to terminal and interstitial regions; no banding was observed at the centromere. Complete banding patterns were established for two of the cultivars examined. The amount of banding per total chromosome complement of these cultivars was approximately 40% and 28%. The results demonstrated the existence of a wide range in the amount of constitutive heterochromatin as measured by the amount of banding between cultivars of similar and different species origins. The banding obtained is discussed with respect to the nature of the heterochromatin exhibited.
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Comings, D. E.: Heterochromatin of the Indian muntjac. Exp. Cell Res. 67, 441–460 (1971)
Comings, D. E.: Biochemical mechanisms of chromosome banding and color banding with Acridine Orange. Nobel Symposium 23, 293–299 (1973)
Daniel, A., Lam-Po-Tang, P. R. L. C.: Mechanism for the chromosome banding phenomenon. Nature (Lond.) 244, 358–359 (1973)
Fredga, K., Mandahl, N.: Autosomal heterochromatin in some Carnivores. Nobel Symposium 23, 104–117 (1973)
Gill, B. S., Kimber, G.: The Giemsa C-banded karyotype of rye. Proc. nat. Acad. Sci. (Wash.) 71, 1247–1249 (1974)
Hsu, T. C.: Constitutive heterochromatin (C-Band) technique. Nobel Symposium 23, 32–33 (1973a)
Hsu, T. C.: Longitudinal differentiation of chromosomes. Ann. Rev. Genet. 7, 153–176 (1973b)
Kho, Y. O., Baër, J.: Incompatibility problems in species crosses of tulips. Euphytica (Wageningen) 20, 30–35 (1971)
Lubs, H. A., McKenzie, W. H., Merrick, S.: Comparative methodology and mechanisms of banding. Nobel Symposium 23, 315–322 (1973)
Marks, G. E., Schweizer, D.: Giemsa Banding: Karyotype differences in some species of Anemone and in Hepatica nobilis. Chromosoma (Berl.) 44, 405–416 (1974)
McKay, R. D. G.: The mechanism of G and C banding in mammalian metaphase chromosomes. Chromosoma (Berl.) 44, 1–14 (1973)
McKenzie, W. H., Lubs, H. A.: An analysis of the technical variables in the production of C bands. Chromosoma (Berl.) 41, 175–183 (1973)
Merker, A.: A Giemsa technique for rapid identification of chromosomes in Triticale. Hereditas (Lund) 75, 280–282 (1973)
Merritt, J. F., Burns, J. A.: Chromosome banding in Nicotiana otophora without denaturation and renaturation. J. Hered. 65, 101–103 (1974)
Pathak, S., Hsu, T. C., Arrighi, F. E.: Chromosomes of Peromyscus (Rodentia, Cricetidae). IV. The role of heterochromatin in karyotypic evolution. Cytogenet. Cell Genet. 12, 315–326 (1973)
Sarma, N. P., Natarajan, A. T.: Identification of heterochromatic regions in the chromosomes of rye. Hereditas (Lund) 74, 233–238 (1973)
Schnedl, W.: Giemsa banding, Quinacrine fluorescence and DNA-replication in chromosomes of cattle (Bos taurus). Chromosoma (Berl.) 38, 319–328 (1972)
Schweizer, D.: Differential staining of plant chromosomes with Giemsa. Chromosoma (Berl.) 40, 307–320 (1973)
Sinha, A. K., Kakati, S., Pathak, S.: Exclusive localization of C-bands with opossum sex chromosomes. Exp. Cell Res. 75, 265–268 (1972)
Stack, S. M., Clarke, C. R.: Pericentromeric chromosome banding in higher plants. Canad. J. Genet. Cytol. 15, 367–369 (1973)
Stack, S. M., Clarke, C. R., Cary, W. E., Muffly, J. T.: Different kinds of heterochromatin in higher plant chromosomes. J. Cell Sci. 14, 499–504 (1974)
Sumner, A. T.: A simple technique for demonstrating centromeric heteroehromatin. Exp. Cell Res. 75, 304–306 (1972)
Vass, L., Sellyei, M.: Heated Giemsa solution for producing more consistent bands on mammalian chromosomes. Humangenetik 18, 81–83 (1973)
Vosa, C. G., Marchi, P.: Quinacrine fluorescence and Giemsa staining in plants. Nature (Lond.) New Biol. 237, 191–192 (1972)
Vosa, C. G.: The enhanced and reduced quinacrine fluorescence bands and their relationship to the Giemsa patterns in Allium flavum. Nobel Symposium 23, 156–158 (1973)
Yamasaki, N.: Differentielle Darstellung der Metaphasechromosomen von Cypripedium debile mit Chinacrin and Giemsa-Färbung. Chromosoma (Berl.) 41, 403–412 (1973)
Yunis, J. J., Sanehez, O.: G-banding and chromosome structure. Chromosoma (Berl.) 44, 15–23 (1973)
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Filion, W.G. Differential Giemsa staining in plants. Chromosoma 49, 51–60 (1974). https://doi.org/10.1007/BF00284987
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DOI: https://doi.org/10.1007/BF00284987