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C banding in polytene chromosomes of Simulium ornatipes and S. melatum (Diptera: Simuliidae)

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Abstract

Polytene and mitotic chromosomes of Simulium ornatipes and S. melatum were subjected to C banding procedures. In both species polytene chromosomes consistently show C banding of centromere regions, telomeres, nucleolar organiser and, unexpectedly, numerous interstitial sites. The interstitial C banding sites correspond to morphologically single polytene bands. Their response is graded and independent of band size. Interstitial C bands in S. ornatipes are scattered throughout the complement, whereas in S. melatum they are clustered. Supernumerary heterochromatic segments in S. ornatipes also exhibit strong C banding and inverted segments can differ from standard in C banding pattern. — Mitotic chromosomes of both species show a single centric C band with indications of two weak interstitial bands in S. ornatipes, suggesting that many C band regions, detectable in polytene chromosomes, are not resolved by present techniques in mitotic chromosomes. — Contrary to current opinion that C banding is diagnostic for constitutive heterochromatin, the interstitial C band sites of polytene chromosomes are regarded as euchromatic. Conversely, the heterochromatic pericentric regions of S. ornatipes are not C banded. — It appears that polytene chromosomes offer a promising system for the elucidation of C banding mechanisms.

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Authors and Affiliations

  1. Department of Population Biology, Research School of Biological Sciences, Australian National University, P. O. Box 475, 2601, Canberra City, A. C. T., Australia

    Daniel G. Bedo

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  1. Daniel G. Bedo
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Bedo, D.G. C banding in polytene chromosomes of Simulium ornatipes and S. melatum (Diptera: Simuliidae). Chromosoma 51, 291–300 (1975). https://doi.org/10.1007/BF00284822

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  • DOI: https://doi.org/10.1007/BF00284822

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