Virus Genes

, Volume 12, Issue 3, pp 265–274

Hamster polyomavirus-encoded proteins: Gene cloning, heterologous expression and immunoreactivity

  • Rainer Ulrich
  • Kirsten Sommerfeld
  • Anett Schröder
  • Helmuth Prokoph
  • Wolfgang Arnold
  • Detlev H. Krüger
  • Siegfried Scherneck


The hamster polyomavirus (HaPV) is associated with spontaneously appearing skin epithelioma of the Syrian hamster Z3 strain. Virus particles prepared from the skin epithelioma cause lymphoma and leukemia when injected into newborn hamsters from a distinct Syrian hamster colony (HaP); in contrast to the skin epithelioma the hemopoietic tumors are virus free but accumulate viral DNA. To study the humoral immune response of HaPV-infected Z3 hamsters we produced recombinant HaPV proteins in Escherichia coli as β-galactosidase-, TrpE-and dihydrofolate reductase-fusion proteins or as non-fused proteins. Recombinant plasmids carried segments of all putative early and late HaPV proteins. The recombinant proteins were detected in stained SDS polyacrylamide gels and in Western blots using monoclonal anti-TrpE and anti-β-galactosidase antibodies and sera of HaPV-infected hamsters.

Sera from HaPV-infected Z3 hamsters and crude lysates of all clones were applied to Western blots to characterize the humoral immune response in the animals. HaPV-specific antibodies were found to be directed against early protein segments translated from the first common exon and from the second unique exon of LT and MT, resp., as well as against the late proteins VP1 and VP2/3. The almost complete VP2 was recognized by all sera whereas VP1 was detected only by a half of the sera. Our data suggest the presence of at least 2 immunodominant regions in VP2, one in the C-terminal VP1 and at least 4 in early proteins.

Key words

hamster polyomavirus heterologous expression epitope localization 


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  1. 1.
    Graffi A., Schramm T., Bender E., Bierwolf D., and Graffi I., Arch Geschwulstforsch 30, 277–283, 1967.Google Scholar
  2. 2.
    Graffi A., Bender E., Schramm T., Graffi I., and Bierwolf D., Comp Leukemia Res Bibl Haematol (Basel), 36, 293–303, 1970.Google Scholar
  3. 3.
    Scherneck S., Delmas V., Vogel F., and Feunteun J., J Virol 61, 3992–3998, 1987.Google Scholar
  4. 4.
    Zimmermann W., Krause H., Scherneck S., Feunteun J., and Geissler E., Gene 29, 243–246, 1984.Google Scholar
  5. 5.
    Delmas V., Bastien C., Scherneck S., and Feunteun J., EMBO J, 4, 1279–1286, 1985.Google Scholar
  6. 6.
    Vogel F., Rhode K., Scherneck S., Bastien C., Delmas V., and Feunteun J., Virology 154, 335–343, 1986.Google Scholar
  7. 7.
    de LaRoche Saint André C., Mazur S., and Feunteun J. J Virol 67, 7172–7180, 1993.Google Scholar
  8. 8.
    Goutebroze L. and Feunteun J., J Virol 66, 2495–2504, 1992.Google Scholar
  9. 9.
    de LaRoche Saint André C., Delmas V., Bastien C., Goutebroze L., Scherneck S., and Feunteun J. in Villarreal L.P. (ed). Common Mechanisms of Transformation by Small DNA Tumor Viruses. Amer Soc Microbiol. Washington DC, 1989, pp. 225–238.Google Scholar
  10. 10.
    Koerner T.J., Hill J.E., Myers A.M., and Tzagoloff A. in Guthrie C. and Fink G.R. (eds), Methods in Enzymology, Vol. 194, Academic Press, San Diego, New York, Boston, London, Sydney, Tokyo, Toronto, 1991, pp. 477–490.Google Scholar
  11. 11.
    Bröker M., Gene Anal Techn 3, 53–57, 1986.Google Scholar
  12. 12.
    Sambrook J., Fritsch E.F., and Maniatis T., Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 1989.Google Scholar
  13. 13.
    Spindler K.R., Rosser D.S.E., and Berk A.J. J Virol 49, 132–141, 1984.Google Scholar
  14. 14.
    Laemmli U.K., Nature 227, 680–685, 1970.PubMedGoogle Scholar
  15. 15.
    Burnette W.N., Anal Biochem 112, 195–203, 1981.Google Scholar
  16. 16.
    Siakkou H., Jahn S., Kienzle N., Ulrich R., Grötzinger C., Schneider T., Kohleisen B., Pauli G., Spohn R., Jung G., Müller-Lantzsch N., and Krüger D.H., Arch Virol 128, 81–95, 1993.Google Scholar
  17. 17.
    Berzins I., Ulrich R., Meisel H., Krüger D.H., Borisova G., Pumpens P., Grens E., Gelderblom H.R., Ladhoff A.-M., Schnitzler P., Darai G., and Bautz E.K.F., in Norrby E., Brown F., Chanock R.M., and Ginsberg H.S. (eds) Vaccines 94. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 1994, pp. 301–308.Google Scholar
  18. 18.
    Courtneidge S.A., Goutebroze L., Cartwight A.., Heber A., Scherneck S., and Feunteun J., J Virol 65, 3301–3308, 1991.Google Scholar
  19. 19.
    Hopp T.P. and Woods K.R., Proc Natl Acad Sci USA 78, 3824–3828, 1981.Google Scholar
  20. 20.
    Shearer M.H., Bright R.K., Lanford R.E., and Kennedy R.C., Clin Exp Immunol 91, 266–271, 1993.Google Scholar
  21. 21.
    Tevethia S.S., Epler M., Georgoff I., Teresky A., Marlow M., and Levine A.J., Virology 190, 459–464, 1992.Google Scholar
  22. 22.
    Bollag B. and Frisque R.J., Virus Res 25, 223–239, 1992.Google Scholar
  23. 23.
    Schoeffel A., Weist S., Ball R.K., Scheidtmann K.H., Braun D.G., and Brandner G., Virology 166, 245–247, 1988.Google Scholar
  24. 24.
    Hess R., Oberbeck J., Grussenmeyer T., Schoeffel-Keller A., Haessler C., Braun D.G., and Brandner G., Intervirology 37, 47–52, 1994.Google Scholar
  25. 25.
    Sawai E.T. and Butel J.S., Virology 189, 782–785, 1992.Google Scholar
  26. 26.
    Bright R.K., Shearer M.H., and Kennedy R.C., Clin Exp Immunol 96, 491–499, 1994.Google Scholar
  27. 27.
    Babe L.M., Brew H., Matsuura S.E., and Scott W.A., J Biol Chem 264, 2665–2671, 1989.Google Scholar
  28. 28.
    Kieny M.P., Gautier C., Tomasetto C., Kuhn I., Hareuveni M., Clertand P., and Lathe R., Int J Cancer 45, 185–189, 1990.Google Scholar
  29. 29.
    Ramquist T., Reinholdsson G., Carlquist M., Bergman T., and Dalianis T., Virology 172, 359–362, 1989.Google Scholar

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Rainer Ulrich
    • 1
  • Kirsten Sommerfeld
    • 1
  • Anett Schröder
    • 2
  • Helmuth Prokoph
    • 2
  • Wolfgang Arnold
    • 3
  • Detlev H. Krüger
    • 1
  • Siegfried Scherneck
    • 2
  1. 1.Institut für Medizinische VirologieUniversitätsklinikum Charité der Humboldt-UniversitätBerlinGermany
  2. 2.Abt. TumorgenetikMax Delbrück Centrum für Molekulare MedizinBerlinGermany
  3. 3.Abt. Med. Onkologie &TumorimmunologieMax Delbrück Centrum für Molekulare MedizinBerlinGermany

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