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A highly sensitive peroxidase-antiperoxidase method for detection of H-Y antigen on cultivated human fibroblasts

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Summary

A peroxidase-anti-peroxidase method for the detection of H-Y antigen at the single cell level is described. The efficiency of the test was examined in cultivated fibroblasts derived from control subjects and from XX males and a true hermaphrodite. For comparison, H-Y antigen was determined in blood cells of the same probands using the cytotoxic test. The finding of H-Y positive fibroblasts in the intersex patients has implications for the origin of these disorders.

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Authors and Affiliations

  1. Institut für Humangenetik und Anthropologie der Universität, Albertstr.11, D-7800, Freiburg, Federal Republic of Germany

    U. Müller

  2. Medizinische Universitätsklinik, Hugstetterstr. 55, D-7800, Freiburg, Federal Republic of Germany

    K. Bross

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  1. U. Müller
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  2. K. Bross
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Müller, U., Bross, K. A highly sensitive peroxidase-antiperoxidase method for detection of H-Y antigen on cultivated human fibroblasts. Hum Genet 52, 143–147 (1979). https://doi.org/10.1007/BF00284609

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  • DOI: https://doi.org/10.1007/BF00284609

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