Summary
The frequency of sister chromatid exchanges (SCEs) was studied in cells from three freshly established lymphoma lines, derived from two patients with Hodgkin's disease and one patient with non-Hodgkin lymphoma. These values were compared to SCE rates found in cells from two long-established lymphoma lines (Raji and BJAB) and to those recorded in control cell lines of normal human donors. The highest SCE levels were demonstrated in the freshly established lymphoma lines, the lowest SCE values separated the lymphoblastoid cell lines from healthy controls, and the older lymphoma lines Raji and BJAB presented rates in between. The influences of BUDR concentration and of the duration of BUDR treatment on the frequency of SCEs were tested. Furthermore, the dependence of the SCE rate on the time interval between establishment of the cell line and its SCE investigation was considered. The connection between elevated SCE rates and the neoplastic nature of lymphoma lines is discussed.
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Bartram, C. R., Koske-Westphal, T., Passarge, E.: Chromatid exchanges in ataxia telangiectasia, Bloom syndrome, Werner syndrome, and Xeroderma pigmentosum. Ann. Hum. Genet. 40, 79–86 (1976)
Chaganti, R. S. K., Schonberg, S., German, J.: A manyfold increase in sister chromatid exchanges in Bloom's syndrome lymphocytes. Proc. Natl. Acad. Sci. USA 71, 4508–4512 (1974)
Cheng, W. S., Mulvihill, J. J., Greene, M. H., Pickle, L. W., Tsai, S., Whang-Peng, J.: Sister chromatid exchanges and chromosomes in chronic myelogenous leukemia and cancer families. Int. J. Cancer 23, 8–13 (1979)
Galloway, S. M., Evans, H. J.: Sister chromatid exchange in human chromosomes from normal individuals and patients with ataxia telangiectasia. Cytogenet. Cell Genet. 15, 17–29 (1975)
Gatti, M., Olivieri, G.: The effect of X-rays on labelling pattern of M1 and M2 chromosomes in Chinese hamster cells. Mutat. Res. 17, 101–112 (1973)
Gebhart, E., Kappauf, H.: Bleomycin and sister-chromatid exchange in human lymphocyte chromosomes. Mutat. Res. 58, 121–124 (1978)
Jarvis, J. E., Ball, G., Rickinson, A. B., Epstein, M. A.: Cytogenetic studies on human lymphoblastoid cell lines from Burkitt's lymphomas and other sources. Int. J. Cancer 14, 716–721 (1974)
Kakati, S., Abe, S., Sandberg, A. A.: Sister chromatid exchange in Philadelphia Chromosome (Ph1)-positive leukemia. Canc. Res. 38, 2918–2921 (1978)
Kato, H., Stich, H. F.: Sister chromatid exchanges in ageing and repair-deficient human fibroblasts. Nature 260, 447–448 (1976)
Klein, G., Lindahl, T., Jondal, M., Leibold, W., Menezes, J., Nilsson, K., Sundström, C.: Continuous lymphoid cell lines with characteristics of B cells (bone-marrow derived), lacking the Epstein-Barr virus genome and derived from three human lymphomas. Proc. Natl. Acad. Sci. USA 71, 3283–3286 (1974)
Knuutila, S., Helminen, E., Vuopio, P., de la Chapelle, A.: Sister chromatid exchanges in human bone marrow cells. I. Control subjects and patients with leukaemia. Hereditas 88, 189–196 (1978)
Kurvink, K., Bloomfield, C. D., Keenan, K. M., Levitt, S., Cervenka, J.: Sister chromatid exchange in lymphocytes from patients with malignant lymphoma. Hum. Genet. 44, 137–144 (1978)
Lambert, B., Ringborg, U., Harper, E., Lindblad, A.: Sister chromatid exchanges in lymphocyte cultures of patients receiving chemotherapy for malignant disorders. Cancer Treatment Rep. 62, 1413–1419 (1978)
Latt, S. A.: Sister chromatid exchanges, indices of human chromosome damage and repair: Detection by fluorescence and induction by Mitomycin C. Proc. Natl. Acad. Sci. USA 71, 3162–3166 (1974)
Latt, S. A., Stetten, G., Juergens, L. A., Buchanan, G. R., Gerald, P. S.: The induction by alkylating agents of sister chromatid exchanges and chromatid breaks in Fanconi's anemia. Proc. Natl. Acad. Sci. USA 72, 4066–4070 (1975)
Michalová, K., Málková, J., Činátl, J., Placerová, J.: Chromosomal characteristics and nonrandom distribution of sister chromatid exchanges in lymphoblastoid cell lines isolated from acute leukemias. Neoplasma 24, 537–545 (1977)
Nilsson, K., Pontén, J.: Classification and biological nature of established human hematopoietic cell lines. Int. J. Cancer 15, 321–341 (1975)
Perry, P., Wolff, S.: New Giemsa method for the differential staining of sister chromatids. Nature 251, 156–158 (1974)
Perry, P., Evans, H. J.: Cytological detection of mutagen-carcinogen exposure by sister chromatid exchange. Nature 258, 121–125 (1975)
Pulvertaft, R. J. V.: A study of malignant tumors in Nigeria by short-term tissue culture. J. Clin. Pathol. 18, 261–273 (1965)
Raposa, T.: Sister chromatid exchange studies for monitoring DNA damage and repair capacity after cytostatics in vitro and in lymphocytes of leukaemic patients under cytostatic therapy. Mutat. Res. 57, 241–251 (1978)
Schaadt, M., Fonatsch, C., Kirchner, H., Diehl, V.: Establishment of a malignant, Epstein-Barr virus (EBV)-negative cell line from the pleura effusion of a patient with Hodgkin's disease. Blut 38, 185–190 (1979a)
Schaadt, M., Kirchner, H., Fonatsch, C., Diehl, V.: Intracranial heterotransplantation of human hematopoietic cells in nude mice. Int. J. Cancer 23, 751–761 (1979b)
Shiraishi, Y., Sandberg, A. A.: Effects of various chemical agents on sister chromatid exchanges, chromosome aberrations, and DNA repair in normal and abnormal human lymphoid cell lines. J. Natl. Cancer Inst. 62, 27–33 (1979)
Solomon, E., Bobrow, M.: Sister chromatid exchanges—A sensitive assay of agents damaging human chromosomes. Mutat. Res. 30, 273–278 (1975)
Stoll, C., Borgaonkar, D. S., Levy, J. M.: Effect of vincristine on sister chromatid exchanges of normal human lymphocytes. Cancer Res. 36, 2710–2713 (1976)
Wolff, S., Rodin, B., Cleaver, J. E.: Sister chromatid exchanges induced by mutagenic carcinogens in normal and Xeroderma pigmentosum cells. Nature 265, 347–349 (1977)
Zech, L., Haglund, U., Nilsson, K., Klein, G.: Characteristic chromosomal abnormalities in biopsies and lymphoid-cell lines from patients with Burkitt and non-Burkitt lymphomas. Int. J. Cancer 17, 47–56 (1976)
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Fonatsch, C., Schaadt, M. & Diehl, V. Sister chromatid exchange in cell lines from malignant lymphomas (lymphoma lines). Hum Genet 52, 107–118 (1979). https://doi.org/10.1007/BF00284604
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DOI: https://doi.org/10.1007/BF00284604