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Orosomucoid (ORM) typing by print lectinofixation: a new technique for isoelectric focusing. Two common alleles in Japan

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Summary

The genetic types of orosomucoid (ORM) were analyzed by isoelectric focusing (IEF) on polyacrylamide gels and subsequent print lectinofixation with a lectin from the beetle, allo A. In this paper, the newly devised print lectinofixation for ORM typing is described. This technique is faster, easier to perform, and has been found to be a useful tool in population genetics and forensic medicine. The results of typing for two alleles, ORM *1 and ORM *2 are described for a population of Northern Japan (n=500).

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Authors and Affiliations

  1. Department of Forensic Medicine, Yamagata University School of Medicine, 990-23, Yamagata, Japan

    K. Umetsu, N. Ikeda, S. Kashimura & T. Suzuki

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  1. K. Umetsu
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  2. N. Ikeda
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  3. S. Kashimura
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  4. T. Suzuki
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Additional information

We use the designation “lectinofixation” to denote the method using lectin in place of monospecific antibody in the immunofixation

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Umetsu, K., Ikeda, N., Kashimura, S. et al. Orosomucoid (ORM) typing by print lectinofixation: a new technique for isoelectric focusing. Two common alleles in Japan. Hum Genet 71, 223–224 (1985). https://doi.org/10.1007/BF00284578

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  • DOI: https://doi.org/10.1007/BF00284578

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