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Effect of different sex of tumour bearing mice and of ovariectomy on the proliferation kinetics of a solid, transplantable mammary carcinoma (C3H-mouse)

Einfluß des Geschlechts tumortragender Mäuse sowie der Ovariektomie auf die Proliferationskinetik eines soliden, transplantablen Mammarcarcinoms der C3H-Maus

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Summary

The aim of the present investigation was to study the influence of sex of tumour bearing mice and of ovariectomy on the proliferation kinetics of a solid, transplantable mammary carcinoma (C3H-mouse). According to the growth curves the tumour growth was delayed in spayed female and in male mice compared with normal female animals. This was due to a longer cell cycle time of tumour cells in the former, mainly caused by a prolonged presynthetic gap (TG1). Concerning the growth fraction and the cell loss factor, essential differences could not be detected. Thus, the solid tumour displayed a distinct though partial disengagement of growth from hormonal dependence.

Zusammenfassung

Das Ziel der vorliegenden Untersuchungen war es, den Einfluß des Geschlechts der tumortragenden Mäuse und der Ovariektomie auf das Wachstumsverhalten eines soliden transplantablen Mammacarcinoms der C3H-Maus zu prüfen. Dabei konnte durch Wachstumskurven ermittelt werden, daß das Wachstum des Tumors bei Transplantation auf ovariektomierte und männliche Tiere gegenüber dem bei weiblichen Tieren verzögert war. Autoradiographisch ließ sich hierbei eine längere Generationszeit der Tumorzellen ermitteln, die vor allem durch eine längere präsynthetische Ruhephase (T G1) bedingt war. In bezug auf die Wachstumsfraktion und den Zellverlustfaktor wurden keine wesentlichen Unterschiede gefunden. Insgesamt ließ sich nur eine eingeschränkte hormonelle Beeinflussung des Wachstums der Tumorzellen feststellen, was u.a. mit dem niedrigen Gehalt an Oestrogenreceptoren zusammenhängen könnte.

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Abbreviations

T Dpot :

potential tumour doubling time

T Dexp :

exponential tumour doubling time

T Deff :

effective tumour doubling time

LI :

labeling index

MI :

mitotic index

T C :

cell cycle time

T S :

DNS-synthesis time

T G1 :

presynthetic gap

T G2 :

postsynthetic gap

GF:

growth fraction

Φ :

cell loss factor

References

  • Barett,J.C.: A mathematical model of the mitotic cycle and its application to the interpretation of percentage labeled mitoses data. J. nat. Cancer Inst. 37, 443–450 (1966)

    Google Scholar 

  • Bresciani,F., Nola,E., Bica,V., Puca,G.A.: Early stages in estrogen control of gene expression and its derangement in cancer. Fed. Proc. 32, 2126–2132 (1973)

    Google Scholar 

  • Denekamp,J.: The cellular proliferation kinetics of animal tumours. Cancer Res. 30, 393–400 (1970)

    Google Scholar 

  • Farris,E.J., Griffith,J.Q. (Ed): The rat in laboratory investigation, p. 448. New York: Hafner Publ. Com. 1963

    Google Scholar 

  • Féaux de Lacroix,W., Clemens,A., Klein,P.J., Castrup,H.J., Lennartz,K.J.: Einfluß der Urämie auf die Wachstumskinetik eines soliden, transplantablen Tumors. Beitr. Path. 155, 410–427 (1975)

    Google Scholar 

  • Féaux de Lacroix,W., Klein, P.J., Klein, H.O., Lennartz, K.J.: Vergleichende autoradiographische Untersuchungen an einem teils differenzierten und einem an aplastischen transplantablen Mammacarcinom der Maus. Beitr. path. Anat. 150, 287–297 (1973)

    Google Scholar 

  • Hempel,E.: Analyse des Zellzyklus der Melanocyten des Harding-Passey-Melanoms der Maus unter besonderer Berücksichtigung der “Growth-Fraction”. Virchows Arch. Abt. B. 1, 15–30 (1968)

    Google Scholar 

  • Löbbecke,E.A., Schultze,B.: Autoradiographische Bestimmung der Generationsdauer verschiedener Zellarten der fetalen und adulten Ratte nach Dauerinfusion von 3H-Thymidin. Verh. Dtsch. Ges. Zool. 30 Suppl. 581–587 (1966)

    Google Scholar 

  • Maurer-Schultze,B.: Methoden zur Zellzyklusanalyse: In: Aktuelle Probleme der Therapie maligner Tumoren (ed. G. Wüst), p. 155–168. G.Thieme Verlag (1973)

  • Mc.Guire,W.L., Chamness,G.C, Costlow,M.E., Shepherd, R.E.: Hormone dependence in breast cancer. Metabolism 23, 75–100 (1974)

    Google Scholar 

  • McGuire,W.L., Julian,J.A., Chamnes,G.C.: A dissociation between ovarian dependent growth and estrogen sensitivity in mammary carcinoma. Endocrinology 89, 696–973 (1971)

    Google Scholar 

  • Mendelsohn,M.L., Dohan,F.C., Moore,H.A.: Autoradiographic analysis of cell proliferation in spontaneous breast cancer of C3H mouse. I. Typical cell cycle and timing of DNS synthesis. J. nat. Cancer Inst. 25, 477–484 (1960a)

    Google Scholar 

  • Mendelsohn,M.L.: Autoradiographische analysis of cell proliferation in spontaneous breast cancer of C3H mouse. II. Growth and survival of cells labeled with tritiated thymidine. J. nat. Cancer Inst. 25, 484–500(1960b)

    Google Scholar 

  • Mendelsohn,M.L.: Autoradiographic analysis of cell proliferation in spontaneous breast concer of C3H mouse. III. The growth fraction J. nat. Cancer Inst. 28, 1015–1029 (1962)

    Google Scholar 

  • Mühlbock,O.: Studies on the hormone dependence of experimental breast tumors in mice. In: Endocrine aspects of breast cancer (ed. A.R.Currie, p. 291–296. Williams and Wilkins Co.; Baltimore (1958)

    Google Scholar 

  • Puca,G.A., Bresciani,F.: Decrease to complete loss of ability to bind estradiol by a soluble macromolecular fraction of C3H mammary tumors. Europ. J. Cancer 3, 475–479 (1968)

    Google Scholar 

  • Quastler, H., Sherman, F.G.: Cell population kinetics in the intestinal epithelium of the mouse. Exp. Cell Res. 17, 420–438, (1959)

    Google Scholar 

  • Sachs,L.: Statistische Auswertungsmethoden. Berlin, Heidelberg, New York: Springer 1968

    Google Scholar 

  • Scatchard,G.: The attractions of proteins for small proteins and ions. Ann. N.Y. Acad. Sci. 51, 660–672 (1949)

    Google Scholar 

  • Simpson-Herren,L., Griswold,D. P.: Studies of the kinetics of growth and regression of 7,12-Dimethylbenz (a) anthrancene induced mammary adenocarcinoma in Sprague-Dawley rats. Cancer Res. 30, 813–818 (1970)

    Google Scholar 

  • Simpson-Herren,L., Lloyd,H.H.: Kinetic parameters and growth curves for experimental tumor systems. Cancer Chemother. Rep. 54, 143–174 (1970)

    Google Scholar 

  • Steel,G.G.: Cell loss of experimental tumors. Cell tiss. Kinet. 1, 193–207 (1968)

    Google Scholar 

  • Steel,G.G.: Personal Communication (1975)

  • Steel,G.G., Adams,K., Barett,J.C: Analysis of the cell population kinetics of transplanted tumors of widely-differing growth rate Brit. J. Cancer 20, 784–800 (1966)

    Google Scholar 

  • Steel,G.G., Hanes,S.: The technique of labelled mitoses: Analysis by automatic curve fitting. Cell tiss. Kinet. 4, 93–105 (1971)

    Google Scholar 

  • Vincent,P.C., Nicholls,A.: Comparison of the growth of the Ehrlich Ascitestumor in male and female mice. Cancer Res. 27, 1058–65 (1967)

    Google Scholar 

  • Wimber,D.E.: Duration of the nuclear cycle in Tradecantia paludosa root tips as measured with 3H-Thymidine. Amer. J. Bot. 47, 828–834 (1960)

    Google Scholar 

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Authors and Affiliations

  1. Institute of Pathology, University of Cologne, Josef-Stelzmann-Str. 9, D-5000, Köln 41, Federal Republic of Germany

    W.Féaux de Lacroix, Hensen F. J., Klein P. J., Nola E. & Lennartz K. J.

  2. Institute of General Pathology, University of Naples, Federal Republic of Germany

    Nola E.

Authors
  1. W.Féaux de Lacroix
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  2. Hensen F. J.
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  3. Klein P. J.
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  4. Nola E.
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  5. Lennartz K. J.
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Dedicated Prof. Dr. ing. W.Maurer to his 70th birthday

Supported by Deutsche Forschungsgemeinschaft

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de Lacroix, W., Hensen, F.J., Klein, P.J. et al. Effect of different sex of tumour bearing mice and of ovariectomy on the proliferation kinetics of a solid, transplantable mammary carcinoma (C3H-mouse). Z. Krebsforsch. 87, 181–191 (1976). https://doi.org/10.1007/BF00284375

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  • DOI: https://doi.org/10.1007/BF00284375

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