Summary
Two methods of mouse skin transplantation are described. Large skin grafts are placed directly on the fascia of the back and fixed with wound clips. This method is suited primarily for grafting carcinogen treated skin. Small skin grafts are applied to the panniculus carnosus and simply covered with a small piece of “Hansaplast” clipped to the back skin. This method is appropriate in particular for allografting, as rejection for all animals occurs nearly simultaneously. For both methods the mortality rate is very low, the results are excellent and highly reproducible. This is mainly due to the method of fixation which is kept as short as possible, 1 day for the large and 4–5 days for the small grafts, thus allowing an early and continuous inspection of the grafts.
Zusammenfassung
Zwei Methoden der Hauttransplantation bei der Maus werden beschrieben. Die eine dient zur Verpflanzung sehr großer Hautstücke direkt auf die Rückenfascie ohne Erhaltung des Panniculus carnosus, mit anschließender Fixation durch Michel Wundklammern. Diese Methode ist besonders für die Transplantation carcinogenbehandelter Haut geeignet.
Bei der anderen Methode werden kleine Hauttransplantate auf den Panniculus carnosus der Rückenhaut aufgelegt und mit einem Stück Hansaplast abgedeckt, das an der Rückenhaut festgeklammert wird. Diese Technik eignet sich besonders für die Verpflanzung von Allotransplantaten, da die Abstoßung sehr gleichmäßig und für alle Transplantate zeitlich nahe beieinander liegend erfolgt.
Die Vorzüge beider Methoden, wie geringe Mortalität, hohe Erfolgssicherheit und kosmetisch gute Ergebnisse liegen zum großen Teil in der Art der Fixation der Transplantate begründet. Diese erfolgt so kurz wie möglich, für 1 Tag bei großen und 4–5 Tage bei kleinen Transplantaten und erlaubt so früh eine dauernde ungehinderte Beobachtung.
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Diese Arbeit wurde aus Mitteln der Deutschen Forschungsgemeinschaft unterstützt.
Wir danken Frl. B. Kissel, Frl. G. Schilling und Herrn V. Ehemann für ihre ausgezeichnete technische Hilfe.
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Worst, P.K.M., Bauz, R., Wahn, U. et al. Hauttransplantationen zum Studium der chemischen Carcinogenese an der Mäusehaut. Z. Krebsforsch. 80, 307–316 (1973). https://doi.org/10.1007/BF00284366
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DOI: https://doi.org/10.1007/BF00284366