Summary
Cells from an experimental sarcoma produced by inoculation of BHK 21 cells clone 13 cells in hamsters showed many similar ultrastructural features with the original cultured cells. Clear and dark cells, probably representing different metabolic stages of the same tumor cell, were noted. In both tumor and culture cells, type R or H virus-like particles could be detected. The high number of R or H particles observed in tumor cells suggests their participation in the oncogenesis of this neoplasm. The tumor cells contained several dilated endoplasmic reticulum cisternae with round and filamentous R type virus particles. Many tumor cells presented nuclear bodies of the filamentous and granular types.
Zusammenfassung
Zellen eines experimentellen Sarkoms, verursacht im Hamster durch Inoculation von BHK 21 Klon- 13 Zellen, wiesen zahlreiche ultrastrukturelle Eigenschaften auf, die mit den Stamm-Kulturzellen vergleichbar sind. Es wurden klare und dunkle Zellen beobachtet, die möglicherweise aus verschiedenen Stufen des Metabolismus derselben Geschwulstzelle entstanden sind. Sowohl in den Geschwulstzellen wie auch in den Kulturzellen sind R oder H Virus-ähnliche Partikeln festzustellen. Die hohe Anzahl der R oder H Partikel in den Geschwulstzellen läßt vermuten, daß diese an der Onkogenese der Neoplasie beteiligt sind. Die Geschwulstzellen enthielten einige erweiterte Cystomen des endoplasmatischen Reticulums, in denen runde und filamentöse Virus-Partikel des R-Typs festzustellen sind. Viele Geschwulstzellen zeigten Kernkörperchen des filamentösen oder granulären Typs.
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Klein-Szanto, A.J.P., Conti, C.J. & Mayo, J. Ultramorphological features of an experimental hamster sarcoma. Z. Krebsforsch. 80, 277–284 (1973). https://doi.org/10.1007/BF00284361
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DOI: https://doi.org/10.1007/BF00284361