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Induction of tumors in the stomach and nervous system of the ACI/N rat by continuous oral administration of 1-methyl-3-acetyl-1-nitrosourea

Erzeugung von Tumoren in Magen und Nervensystem von ACI/N-Ratten durch orale Gabe von 1-Methyl-3-acetyl-1-nitrosoharnstoff (Ac-MNH)

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Summary

Three groups of ACI/N rats of both sexes received continuous administration of 66 (Group I), 30 (Group II), or 13 (Group III) ppm solution of 1-methyl-3-acetyl-1-nitrosourea (Ac-MNU) in their drinking water.

Tumors were found most frequently in the stomach and nervous system. The former was more frequently found in the male than in the female. The incidence was highest in males of Group II, 28/30 (93%), and lowest in females of Group I, 4/30 (13%). Histologically, all epithelial tumors of the stomach were benign adenomas except 5 adenocarcinomas.

Neurogenous tumors were more frequently seen in the female than in the male. The incidence was highest in females of Group I, 29/30 (97%), and lowest in males of Group III, 6/28 (21 %). The tumors were predominant in the central nervous system, especially in the hemispheres.

Transplantation studies were done in some of these tumors. Although gastric tumors failed to get positive transplantation, all the neurogenous tumors transplanted were positive.

Zusammenfassung

Drei Gruppen von ACI/N-Ratten beiderlei Geschlechts erhielten täglich eine Lösung von 66 (Gruppe I), 30 (Gruppe II) bzw. 13 (Gruppe III) ppm 1-Methyl-3-acetyl-1-nitrosoharnstoff (Ac-MNH) im Trinkwasser. Tumoren traten mit höchster Häufigkeit in Magen und Nervensystem auf. Die Magentumoren-Rate war höher bei männlichen als bei weiblichen Tieren, nämlich 50/58 (86%) vs. 27/90 (33%). Die Tumorhäufigkeit war am höchsten bei Männchen der Gruppe II, nämlich 28/30 (93%), und am niedrigsten bei Weibchen in Gruppe I, nämlich 4/30 (13%). Histologisch waren alle epithelialen Magengeschwülste benigne Adenomen mit Ausnahme von 5 Adenocarcinomen.

Neurogene Tumoren wurden häufiger bei weiblichen Ratten als bei männlichen beobachtet. Die Tumorrate war am höchsten bei Weibchen der Gruppe I, 29/30 (97%), und am niedrigsten bei Männchen der Gruppe III, 6/28 (21%). Die Tumoren traten überwiegend im zentralen Nervensystem auf, speziell in den Hemispheren.

Transplantationsversuche mit einigen dieser Tumoren ergaben positive Transplantationen bei den neurogenen Tumoren, während eine solche der Magentumoren nicht gelang.

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Authors and Affiliations

  1. Department of Chemical Pathology, National Institute of Hygienic Sciences, Kamiyooga, Setagayaku, 158, Tokyo, Japan

    A. Maekawa & S. Odashima

  2. Department of Synthetic Chemistry, National Institute of Hygienic Sciences, Japan

    M. Nakadate

Authors
  1. A. Maekawa
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  2. S. Odashima
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  3. M. Nakadate
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Supported by the Subsidy for Cancer Research from the Ministry of Health and Welfare, and a Grant-in Aid for Cancer Research from the Ministry of Education, Japan.

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Maekawa, A., Odashima, S. & Nakadate, M. Induction of tumors in the stomach and nervous system of the ACI/N rat by continuous oral administration of 1-methyl-3-acetyl-1-nitrosourea. Z. Krebsforsch. 86, 195–207 (1976). https://doi.org/10.1007/BF00284007

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