Summary
The tumours in vitro preserved the essential morphological features of experimental gliomas in situ. Protoplasmic astrocytomas and multiform glioblastomas showed higher grade of anaplasia and dysplasia than the parent neoplastic tissue. All types of glioma exhibited high activity of lactate and glucose-6-phosphate dehydrogenase. Marked differences in the enzymatic activity of the tumour cells were frequently observed. Activity of succinate dehydrogenase was low in all types of gliomas. Acid phosphatase activity differed markedly in all cells and higher activity was found in the cells containing also PAS-positive granules.
Zusammenfassung
In vitro bewahrten die Tumoren die wesentlichen morphologischen Eigenschaften der Gliome in situ. Protoplasmatische Astrocytome und multiforme Glioblastome zeigten einen höheren Grad von Anaplasie und Dysplasie als das Ursprungsgewebe. Alle Gliomtypen wiesen eine hohe Aktivität an Lactate und Glucose-6-Phosphat-Dehydrogenase auf. Häufig wurden ausgesprochene Unterschiede in der enzymatischen Aktivität der einzelnen Tumorzellen beobachtet. In allen Gliomtypen war die Aktivität der Succinat-Dehydrogenase niedrig. Die Aktivität der sauren Phosphatase war in den einzelnen Zellen verschieden, eine höhere Aktivität wurde in denjenigen Zellen gefunden, die auch PAS-positive Granula enthielten.
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Investigation carried out under a grant from PL 480 U.S. Public Health Service Programme. Agreement 227704.
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Renkawek, K., Kroh, H. & Krásnicka, Z. Experimental gliomas cultured in vitro. Morphological and histochemical study. Z. Krebsforsch. 77, 247–256 (1972). https://doi.org/10.1007/BF00283966
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DOI: https://doi.org/10.1007/BF00283966