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18p− Syndrome: an unusual case and diagnosis by in situ hybridization with chromosome 18-specific alphoid DNA sequence

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Summary

A patient with an atypical clinical picture of 18p− syndrome is described. By the in situ hybridization technique we localized the chromosome 18-specific cloned repetitive sequence to metaphase chromosomes of the patient. The predominant hybridization of the probe was found in pericentromeric regions of homologous chromosomes 18. The amount of pericentromeric DNA measured by in situ hybridization differed between homologous chromosomes; and the number of radioactive grains was statistically greater in the normal chromosome 18 than in the aberrant chromosome 18p−. The results indicate that this probe may be useful in clinical cytogenetics for identification of aberrant chromosomes, localization of breakpoints, and studies of C-band DNA polymorphism of chromosome 18.

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References

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Authors and Affiliations

  1. Institute of Pediatrics and Children's Surgery, Ministry of Health of the Russian Soviet Federal Socialist Republic, Taldomskaya str.2, 127412, Moscow, Russia

    Svetlana G. Vorsanova, I. A. Demidova & A. F. Tirskaia

  2. All-Union Research Center of Mental Health, Academy of Medical Sciences of the USSR, Zagorodnoe sh. 2, 113152, Moscow, Russia

    Y. B. Yurov, I. A. Alexandrov & S. P. Mitkevich

Authors
  1. Svetlana G. Vorsanova
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  2. Y. B. Yurov
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  3. I. A. Alexandrov
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  4. I. A. Demidova
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  5. S. P. Mitkevich
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  6. A. F. Tirskaia
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Vorsanova, S.G., Yurov, Y.B., Alexandrov, I.A. et al. 18p− Syndrome: an unusual case and diagnosis by in situ hybridization with chromosome 18-specific alphoid DNA sequence. Hum Genet 72, 185–187 (1986). https://doi.org/10.1007/BF00283945

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  • DOI: https://doi.org/10.1007/BF00283945

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