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Identification of trophoblast in chorionic villi biopsy samples

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Summary

We have investigated a test for rapid discrimination between foetal and maternal origin of chorionic villi biopsy samples. A monoclonal antibody named H315 reacting against a specific antigen present on the surface of foetal trophoblastic cells, plus a double-colour staining technique (FITC + PI), have been used for the identification of foetal cells (H315-positive) and for visualization of nucleate (PI-positive) and anucleate (PI-negative) structures of chorionic villi. This test could be useful in differentiating foetal and maternal cells in chorionic villi biopsy samples currently used for prenatal diagnostic purposes.

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References

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Author notes

  1. Angela E. Covone

    Present address: Laboratory of Molecular Genetics, Giannia Gaslini Institute, via 5 Maggio, I-16148, Genova-Quarto, Italy

Authors and Affiliations

  1. Paediatric Research Unit, The Prince Philip Research Laboratories, Guy's Hospital Medical School, SE19RT, London, UK

    Angela E. Covone & P. M. Johnson

  2. Pregnancy Immunology Group, Department of Immunology, University of Liverpool, P.O. Box 147, L69 3BX, Liverpool, UK

    Angela E. Covone & P. M. Johnson

Authors
  1. Angela E. Covone
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  2. P. M. Johnson
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Covone, A.E., Johnson, P.M. Identification of trophoblast in chorionic villi biopsy samples. Hum Genet 72, 172–173 (1986). https://doi.org/10.1007/BF00283940

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  • DOI: https://doi.org/10.1007/BF00283940

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