, Volume 45, Issue 1, pp 43–62 | Cite as

DNA replication and RNA transcription of euchromatic and heterochromatic chromosome regions during grasshopper meiosis

  • D. P. Fox
  • G. M. Hewitt
  • D. J. Hall


The sequence of chromosome replication and the extent of RNA transcription were determined for pre-meiotic S-phase and first meiotic prophase in males of Myrmeleotettix maculatus and Chorthippus parallelus. Some heterochromatic chromosome segments are replicated early in S-phase while others are replicated late. In general, heavily condensed heterochromatic regions of the chromosomes show little or no RNA transcription as defined by 3H-uridine incorporation. Extra (i.e. in excess of 2) M4 autosomes in Ch. parallelus are slightly more condensed (heterochromatic) than their two homologues and this change in coiling behaviour is paralleled by a reduction in the rate of transcription and a change in the time of its replication, more of the DNA being synthesised at the end of S-phase. These findings are discussed in relation to the definition of heterochromatin.


Developmental Biology Chromosome Region Chromosome Segment Meiotic Prophase Heterochromatic Region 
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  1. Bennett, M. D.: The duration of meiosis. Proc. roy. Soc. (Lond.) B 178, 277–299 (1971)Google Scholar
  2. Bennett, M. D.: Nuclear DNA content and minimum generation time in herbaceous plant. Proc. roy. Soc. (Lond.) B 181, 109–135 (1972)Google Scholar
  3. Brown, S. W.: Heterochromatin. Science 151, 417–425 (1966)Google Scholar
  4. Callan, H. G., Taylor, J. H.: A radioautographic study of the time course of male meiosis in the newt Trituras vulgaris. J. Cell Sci. 3, 615–626 (1968)Google Scholar
  5. Conger, A. D., Fairchild, L. M.: A quick-freeze method for making smear slides permanent. Stain Technol. 28, 281–283 (1953)Google Scholar
  6. Craig-Cameron, T. A.: Asynchronous chromosome replication at the end of premeiotic DNA synthesis in Schistocerca gregaria (Forskål). Cytobios 4, 7–19 (1971)Google Scholar
  7. Fawcett, D. W.: Observations on cell differentiation and organelle continuity in spermatogenesis. Proc. Int. Symp. on the Genetics of the Spermatozoon. (R. A. Beatty and S. Gluecksohn-Waelsch, eds.). Edinburgh-New York 1972Google Scholar
  8. Fox, D. P., Carter, K. C., Hewitt, G. M.: Giemsa banding and Chiasma distribution in the Desert Locust. Heredity 31, 272–276 (1973)Google Scholar
  9. Gallagher, A., Hewitt, G. M., Gibson, I.: Differential Giemsa staining of heterochromatic B-chromosomes in Myrmeleotettix maculatus (Thunb.) (Orthoptera: Acrididae). Chromosoma (Berl.) 40, 167–172 (1973)Google Scholar
  10. Heitz, E.: Das Heterochromatin der Moose. I. Jb. wiss. Bot 69, 762–818 (1928)Google Scholar
  11. Heitz, E.: Heterochromatin, Chromoeentren, Chromomeren. Ber. Dtsch. Bot. Ges. 47, 274–284 (1929)Google Scholar
  12. Henderson, S. A.: RNA synthesis during male meiosis and spermiogenesis. Chromosoma (Berl.) 15, 345–366 (1964)Google Scholar
  13. Hess, O.: Genetic function correlated with unfolding of lambrush loops by the Y-chromosome in spermatocytes of Drosophila hydei. Molec. gen. Genet. 106, 328–346 (1970)Google Scholar
  14. Hewitt, G. M.: On the evolution and maintenance of B-chromosomes with particular reference to Orthopteran systems. Chromosomes Today 4 (in press, 1973)Google Scholar
  15. Hewitt, G. M., Brown, F. M.: The B-chromosome system of Myrmeleotettix maculatus. V. A steep cline in East Anglia. Heredity 25, 363–371 (1970)Google Scholar
  16. Hewitt, G. M., John, B.: The B-chromosome system of Myrmeleotettix maculatus (Thunb.). III. The statistics. Chromosoma (Berl.) 21, 140–162 (1967)Google Scholar
  17. Hewitt, G. M., John, B.: Parallel polymorphism for supernumerary segments in Chorthippus parallelus (Zetterstedt). I. British populations. Chromosoma (Berl.) 25, 319–342 (1968)Google Scholar
  18. Hewitt, G. M., John, B.: The B-chromosome system of Myrmeleotettix maculatus (Thunb.). IV. The dynamics. Evolution (Lawrence, Kans.) 24, 169–180 (1970)Google Scholar
  19. Hewitt, G. M., John, B.: Parallel polymorphism for supernumerary segments in Chorthippus parallelus (Zetterstedt). IV. Ashurst revisited. Chromosoma (Berl.) 31, 198–206 (1970)Google Scholar
  20. John, B., Hewitt, G. M.: The B-chromosome system of Myrmeleotettix maculatus (Thunb.). II. The statistics. Chromosoma (Berl.) 17, 121–138 (1965)Google Scholar
  21. John, B., Hewitt, G. M.: A polymorphism for heterochromatic supernumerary segments in Chorthippus parallelus. Chromosoma (Berl.) 18, 254–271 (1966)Google Scholar
  22. Lewis, K. R., John, B.: The matter of mendelian heredity. London: Churchill 1964Google Scholar
  23. Lima-de-Faria, A.: Differential uptake of tritiated thymidine into hetero- and euchromatin in Melanoplus and Secale. J. biophys. biochem. Cytol. 6, 457–466 (1959)Google Scholar
  24. Lima-de-Faria, A., Jaworska, H.: Late DNA synthesis in heterochromatin. Nature (Lond.) 217, 138–142 (1968)Google Scholar
  25. Muckenthaler, F. A.: Autoradiographic study of nucleic acid synthesis during spermatogenesis in the grasshopper Melanoplus differentialis. Exp. Cell Res. 35, 531–547 (1964)Google Scholar
  26. Nicklas, R. B., Jaqua, B. A.: X-chromosome DNA replication developmental shift from synchrony to asynchrony. Science 147, 1041–1043 (1965)Google Scholar
  27. Saunders, G. F., Shirakawa, S., Saunders, P. P., Arrighi, F. E., Hsu, T. C.: Populations of repeated DNA sequences in the human genome. J. molec. Biol. 63, 323–334 (1972)Google Scholar
  28. Stebbings, A. F. K., Hewitt, G. M.: The laboratory breeding of British Gomphocerine grasshoppers (Acrididae, Orthoptera). Acrida (Paris) 1, 233–245 (1972)Google Scholar
  29. Swanson, C. P., Merz, T., Young, W. J.: Cytogenetics. Bnglewood Cliffs, N.J.: Prentice-Hall 1967Google Scholar
  30. Tanaka, R.: H3-thymidine autoradiographic studies on the heteropycnosis, heterochromatin and euchromatin in Spiranthes sinensis. Bot. Mag. (Tokyo) 78, 50–62 (1965)Google Scholar
  31. Taylor, J. H.: Distribution of tritium-labelled DNA among chromosomes during meiosis. I. Spermatogenesis in the grasshopper. J. Cell Biol. 35, 57–67 (1965)Google Scholar
  32. Westerman, M.: The effect of X-irradiation on male meiosis in Schistocerca gregaria (Forskål). I. Chiasma frequency response. Chromosoma (Berl.) 22, 401–416 (1967)Google Scholar
  33. Westerman, M.: Parallel polymorphism for supernumerary segments in Chorthippus parallelus (Zetterstedt). II. French populations. Chromosoma (Berl.) 26, 7–21 (1969)Google Scholar
  34. Westerman, M.: The effect of X-irradiation on chiasma frequency in Chorthippus brunneus. Heredity 27, 83–91 (1971)Google Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • D. P. Fox
    • 1
    • 2
  • G. M. Hewitt
    • 1
    • 2
  • D. J. Hall
    • 1
    • 2
  1. 1.Department of GeneticsUniversity of AberdeenNorwich
  2. 2.School of Biological SciencesUniversity of East AngliaNorwich

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