Skip to main content
SpringerLink
Log in

Bidirectional replication of the chromosome in Rhizobium trifolii

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Culture of the thymine-dependent mutant of Rhizobium trifolii T37 was synchronized with phenethanol. During bacterial synchronous growth with synchronized replication of DNA, cells were differentially labeled using subsequently 3H-thymidine of low and high specific activity. The grain tracks produced in autoradiographs of chromosomes were denser on both ends than in the middle. In control experiments with bacteria labeled only with 3H-thymidine of low specific activity, the grain density was uniform throughout the grain track. The results constitute clear evidence of bidirectional replication of R. trifolli chromosome.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adelberg, E.A., Mandel, M., Chein Ching Chen, G.: Optimal conditions for mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine in Escherichia coli K12. Biochem. biophys. Res. Commun. 18, 788–795 (1965)

    Google Scholar 

  • Androsov, V.V.: Synchronization of replication cycle of chromosome of Escherichia coli K12 by nalidixic acid: initiation, direction, symmetry, and termination of replication. Dokl. Acad. Sci. SSSR 228, 205–208 (1976) [in Russian]

    Google Scholar 

  • Bird, E.R., Louarn, I., Martuscelli, J., Caro, L.: Origin and sequence of chromosome replication in Escherichia coli. J. molec. Biol. 70, 549–566 (1972)

    Google Scholar 

  • Bollum, F.J.: Filter paper disk techniques for assaying radioactive macromolecules, pp. 169–173 in Methods in enzymology, Vol. 12B L. Grossman, K. Moldave (ed.) New York: Academic Press Inc. 1968

    Google Scholar 

  • Caro, L.G., Berg, C.M.: Chromosome replication in some strains of Escherichia coli K12. Cold Spr. Harb. Symp. quant. Biol. 33, 559–573 (1968)

    Google Scholar 

  • Caster, J.H.: Selection of thymine-requiring strains from Escherichia coli on solid medium. J. Bact. 94, 1804 (1967)

    Google Scholar 

  • Edlund, T., Gustafsson, P., Wolf-Watz, H.: Effect of thymine concentration on the mode of chromosomal replication in Escherichia coli K12. J. molec. Biol. 108, 295–303 (1976)

    Google Scholar 

  • Fujisawa, T., Eisenstark, A.: Bi-directional chromosomal replication in Salmonella typhimurium. J. Bact. 115, 168–176 (1973)

    Google Scholar 

  • Gyurasits, E.B., Wake, R.G.: Bidirectional chromosome replication in Bacillus subtilis. J. molec. Biol. 73, 55–63 (1973)

    Google Scholar 

  • Hara, H., Yoshikawa, H.: Asymmetric bidirectional replication of Bacillus subtilis chromosome. Nature (Lond.) New Biol 244, 200–223 (1973)

    Google Scholar 

  • Harford, N.: Bidirectional chromosome replication in Bacillus subtilis 168. J. Bact. 121, 835–847 (1975)

    Google Scholar 

  • Hohlfeld, R., Vielmetter, W.: Bidirectional growth of the E. coli chromosome. Nature (Lond.) New Biol. 242, 130–132 (1973)

    Google Scholar 

  • Jonasson, J.: Evidence for bidirectional chromosome replication in Escherichia coli C based on marker-frequency analysis by DNA-DNA hybridization with P2 and λ prophages. Molec. gen. Genet. 120, 69–90 (1973)

    Google Scholar 

  • Lane, H.E.D., Denhardt, D.T.: The rep mutation. IV. Slower movement of replication forks in Escherichia coli rep strains. J. molec. Biol. 97, 99–112 (1975)

    Google Scholar 

  • Masters, M., Broda, P.: Evidence for the bidirectional replication of the Escherichia coli chromosome. Nature (Lond.) New Biol. 232, 137–140 (1971)

    Google Scholar 

  • McKenna, W.G., Masters, M.: Biochemical evidence for the bidirectional replication of DNA in Escherichia coli. Nature (Lond.) 240, 536–539 (1972)

    Google Scholar 

  • Nishioka, Y., Eisenstark, A.: Sequence of genes replicated in Salmonella typhimurium as examined by transduction techniques. J. Bact. 102, 320–333 (1970)

    Google Scholar 

  • O'Sullivan, M.A., Howard, K., Sueoka, N.: Location of a unique replication terminus and genetic evidence for partial bidirectional replication in the Bacillus subtilis chromosome. J. molec. Biol. 91, 15–38 (197a)

    Google Scholar 

  • O'Sullivan, M.A., Howard, K., Sueoka, N.: On the bidirectional replication of the Bacillus subtilis chromosome. J. molec. Biol. 99, 347–348 (1975b)

    Google Scholar 

  • Prescott, D.M., Kuempel, P.L.: Bidirectional replication of the chromosome in Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 69, 2842–2845 (1972)

    Google Scholar 

  • Rodriguez, R.L., Dalbey, M.S., Davern, C.I.: Autoradiographic evidence for bidirectional DNA replication in Escherichia coli. J. molec. Biol. 74, 599–604 (1973)

    Google Scholar 

  • Rodriguez, R.L., Davern, C.I.: Direction of deoxyribonucleic acid replication in Escherichia coli under various conditions of cell growth. J. Bact. 125, 346–352 (1976)

    Google Scholar 

  • Rogers, A.W.: Techniques of autoradiography, Amsterdam: Elsevier Sci. Publ. Co. 1973

    Google Scholar 

  • Treick, R.W., Konetzka, W.A.: Physiological state of Escherichia coli and the inhibition of deoxyribonucleic acid synthesis by phenethyl alcohol. J. Bact. 88, 1580–1584 (1964)

    Google Scholar 

  • Vincent, J.M.: A manual for the practical study of root-nodule bacteria. Oxford-Edinburgh: Blackwell Sci. Publ. 1970

    Google Scholar 

  • Wake, R.G.: Circularity of the Bacillus subtilis chromosome and further studies on its bidirectional replication. J. molec. Biol. 77, 569–575 (1973)

    Google Scholar 

  • Zurkowski, W., Lorkiewicz, Z.: Replication and mapping of Rhizobium trifolii chromosome. In publication (1977)

Download references

Author information

Authors and Affiliations

  1. Institute of Microbiology, University of M. Curie-Skłodowska, Akademicka 19, 20-033, Lublin, Poland

    Witold Zurkowski & Zbigniew Lorkiewicz

Authors
  1. Witold Zurkowski
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zbigniew Lorkiewicz
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by W. Gajewski

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zurkowski, W., Lorkiewicz, Z. Bidirectional replication of the chromosome in Rhizobium trifolii . Molec. Gen. Genet. 156, 215–219 (1977). https://doi.org/10.1007/BF00283494

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00283494

Keywords

Search

Navigation