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Isolation and characterization of mutants of Escherichia coli deficient in induction of mutations by ultraviolet light

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Summary

Mutants of E. coli defective in susceptibility to UV-induction of mutations were isolated by direct screening for their UV nonmutable phenotype (Umu−). Screening of about 30,000 mutagenized clones of a uvrB − derivative of AB1157 yielded six Umu− strains. The mutants can be classified into three groups by the location of the mutations, umuA, umuB and umuC. Mutations umuA and umuB are, respectively, mapped close to lexA and recA genes and mutations at both loci partially reduce UV mutagenesis. The locus of umuC is between hemA and purB and the mutations at this new locus result in a moderate increase of UV sensitivity. The mutation diminishes UV mutagenesis and UV reactivation of phage λ without affecting the inducibility of phophage λ nor the inhibition of cell division following UV irradiation. Related properties of an isogenic strain of a recF − mutant are compared with those of umuC −.

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References

  • Bachmann, B.J.: Pedrigrees of some mutant strains of Escherichia coli K-12. Bact. Rev. 36, 525–557 (1972)

    Google Scholar 

  • Bachmann, B.J., Low, K.B., Taylor, A.L.: Recalibrated linkage map of Escherichia coli K-12. Bact. Rev. 40, 116–167 (1976)

    Google Scholar 

  • Blanco, M., Levine, A., Devoret, R.: lexB: a new gene governing radiation sensitivity and lysogenic induction in Escherichia coli K-12. In: Molecular mechanisms for the repair of DNA (Hanawlat, P. and Setlow, R.B., eds.), pp. 379–382. New York: Plenum Press 1975

    Google Scholar 

  • Boyce, R.P., Howard-Flanders, P.: Release of ultraviolet light-induced thymine dimers from DNA in E. coli K-12. Proc. nat. Acad. Sci. (Wash.) 51, 293–300 (1964)

    Google Scholar 

  • Catellazzi, M., George, J., Buttin, G.: Prophage induction and cell division in E. coli. I. Further characterization of the thermosensitive mutation tif-1 whose expression mimics the effect of UV irradiation. Molec. gen. Genet. 119, 139–152 (1972a)

    Google Scholar 

  • Castellazzi, M., George, J., Buttin, G.: Prophage induction and cell division in E. coli. II. Linked (recA zab) and unlinked (lexA) suppressors of tif-1-mediated induction and filamentation. Molec. gen. Genet. 119, 153–174 (1972b)

    Google Scholar 

  • Clark, A.J., Margulies, A.D.: Isolation and characterization of recombination-deficient mutants of Escherichia coli K-12. Proc. nat. Acad. Sci. (Wash.) 53, 451–459 (1965)

    Google Scholar 

  • Defais, M., Caillet-Fauquet, P., Fox, M.S., Radman, M.: Induction kinetics of mutagenic DNA repair activity in E. coli following ultraviolet irradiation. Molec. gen. Genet. 148, 125–130 (1976)

    Google Scholar 

  • Ganesan, A.K., Seawell, P.C.: The effect of lexA and recf mutations on postreplication repair and DNA synthesis in Escherichia coli K-12. Molec. gen. Genet. 141, 189–206 (1975)

    Google Scholar 

  • George, J., Castellazzi, M., Buttin, G.: Prophage induction and cell division in E. coli. III. Mutations sfiA and sfiB restore division in tif and lon strains and permit expression of mutator properties of tif-1. Molec. gen. Genet. 140, 309–332 (1975)

    Google Scholar 

  • Green, M.H.L., Greenberg, J., Donch, J.: Effect of a recA gene on cell division and capsular polysaccharide production in a lon strain of Escherichia coli. Genet. Res. 14, 159–162 (1969)

    Google Scholar 

  • Guest, J.G.: Biochemical and genetic studies with nitrate reductase C-gene mutants of Escherichia coli. Molec. gen. Genet. 105, 285–297 (1969)

    Google Scholar 

  • Horii, Z.I., Clark, A.J.: Genetic analysis of the RecF pathway of genetic recombination in Escherichia coli K-12: isolation and characterization of mutants. J. molec. Biol. 80, 327–344 (1973)

    Google Scholar 

  • Ishii, Y., Kondo, S.: Spontaneous and radiation-induced deletion mutations in Escherichia coli strains with different DNA repair capacities. Mutation Res. 16, 13–25 (1972)

    Google Scholar 

  • Inouye, M.: Pleiotropic effect of the recA gene of Escherichia coli: uncoupling of cell division from DNA replication. J. Bact. 106, 539–542 (1971)

    Google Scholar 

  • Kato, T.: Effects of chloramphenicol and caffeine on postreplication repair in uvrA − Excision repair characteristics of recB − res −and uvrC −strains of Escherichia coli umuC −and uvrA − Excision repair characteristics of recB − res −and uvrC −strains of Escherichia coli recF −strains of Escherichia coli K-12. Molec. gen. Genet. 156, 115–120 (1977a)

    Google Scholar 

  • Kato, T., Rothman, R.H., Clark, A.J.: Analysis of the recombination and repair in mutagenesis of Escherichia coli by UV irradiation. Genetics in press (1977b)

  • Kondo, S.: Mutagenicity versus radiosensitivity in Escherichia coli. Proc. XIIth Intern. Congr. Genetics. Vol. II, pp. 126–127 (1969)

    Google Scholar 

  • Kondo, S., Kato, T.: Photoreactivation of mutationand killing in Escherichia coli. Advanc. biol. med. Phys. 12, 283–298 (1968)

    Google Scholar 

  • Low, K.B.: Escherichia coli K-12 F-prime factors Old and New. Bact. Rev. 36, 587–607 (1972)

    Google Scholar 

  • Lwoff, A., Dimonovitch, L., Kjeldgaard, N.: Induction de la production de bactériophages chez une bactérie lysogéne. Ann. Inst. Pasteur 79, 815–859 (1950)

    Google Scholar 

  • Marsden, H.S., Pollard, E.C., Ginoza, W., Randall, E.P.: Involvement of recA and exr genes in the in vivo inhibition of the recBC nuclease. J. Bact. 118, 465–470 (1974)

    Google Scholar 

  • Miura, A., Tomizawa, J.: Studies on radiation-sensitive mutants of E. coli III. participation of the Rec system in induction of mutation by ultraviolet irradiation. Molec. gen. Genet. 103, 1–10 (1968)

    Google Scholar 

  • Mount, D.W.: A mutant of Escherichia coli showing constitutive expression of the lysogenic induction and error-prone DNA repair pathways. Proc. nat. Acad. Sci. (Wash.) 74, 300–304 (1977)

    Google Scholar 

  • Mount, D.W., Low, K.B., Edmiston, S.J.: Dominant mutations (lex) in Escherichia coli K-12 which affect radiation sensitivity and frequency of ultraviolet light-induced mutations. J. Bact. 112, 886–893 (1972)

    Google Scholar 

  • Mount, D.W., Walker, A.C., Kosel, C.: Suppression of lex mutations affecting deoxyribonucleic acid repair in Escherichia coli K-12 by closely linked thermosensitive mutations. J. Bact. 116, 950–956 (1973)

    Google Scholar 

  • Radman, M.: Phenomenology of an inducible mutagenic DNA repair pathways in Escherichia coli: SOS repair hypothesis. In: Molecular and environmental aspects of mutagenesis (Prakash, L., Sherman, F., Miller, M., Lawrence, C., and Tabor, H.W., eds.), pp. 129–142. Springfield, Ill.: C.C. Thomas 1974

    Google Scholar 

  • Roberts, J.W., Roberts, C.W.: Proteolytic cleavage of bacteriophage lambda repressor in induction. Proc. nat. Acad. Sci. (Wash.) 72, 147–151 (1975)

    Google Scholar 

  • Rothman, R.H., Kato, T., Clark, A.J.: The beginning of an investigation of recF in the pathways of metabolism of ultraviolet-irradiated DNA in Escherichia coli. In: Molecular mechanisms for the repair of DNA (Hanawalt, P.C., and Setlow, R.B. eds.), pp. 283–291. New York: Pleum Press 1975

    Google Scholar 

  • Rupp, W.D., Howard-Flanders, P.: Discontinuities in the DNA synthesized in an excision-defective strain of Escherichia coli following ultraviolet irradiation. J. molec. Biol. 31, 291–304 (1968)

    Google Scholar 

  • Setlow, R.B., Carrier, W.L.: The disappearance of thymine dimers from DNA: an error-correcting mechanism. Proc. nat. Acad. Sci. (Wash.) 51, 226–231 (1964)

    Google Scholar 

  • Smith, K.C., Menu, D.H.C.: Reapair of radiation-induced damage in Escherichia coli. II. Effect of rec mutation on postreplication repair of damage due to ultraviolet radiation. J. molec. Biol. 51, 459–472 (1970)

    Google Scholar 

  • Swenson, P.A., Schenley, R.L.: Respiration, growth and viability of repair-deficient mutants of Escherichia coli after ultraviolet irradiation. Int. J. Radiat. Biol. 25, 51–60 (1974)

    Google Scholar 

  • Tomizawa, J., Ogawa, T.: Effect of ultraviolet irradiation on bacteriophage lambda immunity. J. molec. Biol. 23, 247–263 (1967)

    Google Scholar 

  • Volkert, M.R., George, D.L., Witkin, E.M.: Partial suppression of the LexA phenotype by mutations (rnm) which restore ultraviolet resistance but not ultraviolet mutability to Escherichia coli B/r uvrA lexA. Mutations Res. 36, 17–28 (1976)

    Google Scholar 

  • Weigle, J.J.: Induction of mutation in a bacterial virus, Proc. nat. Acad. Sci. (Wash.) 39, 628–636 (1953)

    Google Scholar 

  • Willetts, N.S., Clark, A.J., Low, K.B.: Genetic location of certain mutations conferring recombination deficiency in Escherichia coli. J. Bact. 97, 244–249 (1969)

    Google Scholar 

  • Witkin, E.M.: Mutation-proof and mutation-rone modes of survival in derivatives of Escherichia coli B differing in sensitivity to ultraviolet light. Brookhaven Symp. Biol. 20, 17–55 (1967)

    Google Scholar 

  • Witkin, E.M.: Ultraviolet-induced mutation and DNA repair. Ann. Rev. Microbiol. 23, 487–514 (1969)

    Google Scholar 

  • Witkin, E.M.: Elevated mutability of polA and uvrA polA derivatives of Escherichia coli B/r at sublethal doses of ultraviolet light: evidence for an inducible error-prone repair system (“SOS repair”) and its anomalous expression in the strains. Genetics 79, 199–213 (1975)

    Google Scholar 

  • Witkin, E.M.: Ultraviolet mutagenesis an inducible DNA repair in Escherichia coli. Bact. Rev. 40, 869–907 (1976)

    Google Scholar 

  • Youngs, D.A., Smith, K.C.: Evidence for the control by exrA and polA genes of two branches of the uvr gene-dependent excision repair pathway in Escherichia coli K-12. J. Bact. 116, 175–182 (1973)

    Google Scholar 

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  1. Department of Fundamental Radiology, Faculty of Medicine, Osaka University, Kita-ku, 530, Osaka, Japan

    Takesi Kato & Yukiko Shinoura

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  1. Takesi Kato
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  2. Yukiko Shinoura
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Communicated by E. Witkin

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Kato, T., Shinoura, Y. Isolation and characterization of mutants of Escherichia coli deficient in induction of mutations by ultraviolet light. Molec. Gen. Genet. 156, 121–131 (1977). https://doi.org/10.1007/BF00283484

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