Summary
A method is described for the selection of deletions in those genes of Escherichia coli that can be carried on specialized transducing phages.
References
Gottesman, M. E., Yarmolinsky, M. B.: Integration-negative mutants of bacteriophage lambda. J. molec. Biol. 31, 487–505 (1968).
Gottesman, S., Beckwith, J. R.: Directed transposition of the arabinose operon: A technique for the isolation of specialized transducing bacteriophages for any Escherichia coli gene. J. molec. Biol. 44, 117–127 (1969).
Kaiser, A. D., Jacob, F.: Recombination between related temperate bacteriophages and the genetic control of immunity and prophage localization. Virology 4, 509–521 (1957).
Matsushiro, A.: Specialized transduction of tryptophan markers in Escherichia coli K12 by bacteriophage ϕ80. Virology 19, 475–482 (1963).
Morse, M. L., Lederberg, E. M., Lederberg, J.: Transduction in Escherichia coli K12. Genetics 41, 142–156 (1956).
Parkinson, J. S., Davis, R. W.: Deletions in the center of the phage lambda chromosome. Proc. nat. Acad. Sci. (Wash.) 61, 1152 (1968).
Russell, R. L., Abelson, J. N., Landy, A., Gefter, M.L., Brenner, S., Smith, J. D.: Duplicate genes for tryrosine transfer RNA in Escherichia coli. J. molec. Biol. 47, 1–13 (1970).
Schwartz, D. O., Beckwith, J. R.: Mutagens which cause deletions in Escherichia coli. Genetics 61, 371–376 (1969).
Shapiro, J., Machattie, L., Eron, L., Ihler, G., Ippen, K., Beckwith, J. R.: Isolation of pure lac operon DNA. Nature (Lond.) 224, 768–774 (1969).
Wollman, E. L.: Transduction specifique du marqueur biotine par le bacteriophage λ. C. Acad. Sci. (Paris) 257, 4225–4226 (1963).
Yanofsky, C., Lennox, E. S.: Transduction and recombination study of linkage relationships among the genes controlling tryptophan synthesis in Escherichia coli. Virology 8, 425–447 (1959).
Author information
Authors and Affiliations
Additional information
Communicated by R. Pritchard
Rights and permissions
About this article
Cite this article
Burdon, M.G. A potential method for selecting genetic deletions in Escherichia coli . Molec. Gen. Genet. 108, 288–290 (1970). https://doi.org/10.1007/BF00283359
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00283359