Summary
Infectious DNA from phage T1 was inactivated by UV-light (2,537 Å). No effect of irradiation on the kinetics of the assay in a spheroplast system could be observed. UV-damaged molecules compete with unirradiated DNA for the infection. Infectious T1-DNA is subject to host-cell reactivation of UV-damage, the amount of which depends on the physiological conditions of the spheroplasts. Though UV-radiosensitivity of T1 particles is not influenced by the presence of the radical scavenging compound cysteamine, infectious DNA can be protected effectively by this chemical (0.01M) against UV-damage when HCR-negative spheroplasts are used for the assay. Incorporation of 5-bromouracil radiosensitizes infectious T1-DNA in the presence and absence of HCR. This effect can be eliminated when the DNA is irradiated in the presence of cysteamine. The mechanism of radioprotection is discussed.
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Communicated by E. Witkin
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Hotz, G., Mauser, R. Infectious DNA from coliphage T1. Molec. Gen. Genet. 108, 233–242 (1970). https://doi.org/10.1007/BF00283353
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DOI: https://doi.org/10.1007/BF00283353