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Molecular and General Genetics MGG

, Volume 245, Issue 2, pp 133–138 | Cite as

Physical mapping of the 5S ribosomal RNA genes on rice chromosome 11

  • Yasuko Kamisugi
  • Shigeki Nakayama
  • Reiko Nakajima
  • Hisako Ohtsubo
  • Eiichi Ohtsubo
  • Kiichi Fukui
Original Paper

Abstract

One 5S ribosomal RNA gene (5S rDNA) locus was localized on chromosome 11 of japonica rice by in situ hybridization. The biotinylated DNA probe used was prepared by direct cloning and direct labeling methods, and the locus was localized to the proximal region of the short arm of chromosome 11 (llpl.l) by imaging methods. The distance between the signal site and the centromere is 4.0 arbitrary units, where the total length of the short arm is 43.3 units. The 5S rDNA locus physically identified and mapped in rice was designated as 5SRrn. The position of the 5S rDNA locus reported here differs from that in indica rice; possible reasons for this difference are discussed. DNA sequences of 5S rDNA are also reported.

Key words

Rice 5S ribosomal RNA gene In situ hybridization Imaging 5S rDNA sequence 

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Yasuko Kamisugi
    • 1
  • Shigeki Nakayama
    • 2
  • Reiko Nakajima
    • 3
  • Hisako Ohtsubo
    • 3
  • Eiichi Ohtsubo
    • 3
  • Kiichi Fukui
    • 4
  1. 1.Cambridge LaboratoryJohn Innes Centre for Plant Science ResearchNorwichEngland
  2. 2.Department of Genetic Resources IINational Institute of Agrobiological ResourcesTsukubaJapan
  3. 3.Institute of Molecular and Cellular BiosciencesThe University of TokyoTokyoJapan
  4. 4.Department of Crop BreedingHokuriku National Agricultural Experiment StationJoetsuJapan

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